Nair V Siddharth, Nayak Moksha, Ramya M K, Sivadas G, Ganesh C, Devi S Lakshmi, Vedam Vaishnavi
Department of Conservative Dentistry and Endodontics, Sri Sankara Dental College, Trivandrum, Kerala, India.
Department of Conservative Dentistry and Endodontics, KVG Dental College, Sullia, Karnataka, India.
J Pharm Bioallied Sci. 2017 Nov;9(Suppl 1):S41-S44. doi: 10.4103/jpbs.JPBS_92_17.
The aim of this study is to detect adherence of to the infected dentinal tubules of human extracted teeth using confocal laser scanning microscope.
Roots from human premolar teeth ( = 40) were infected with strain the American Type Culture Collection 29212 in brain heart infusion for 21 days. After the experimental period, specimens were divided into two groups, Group A ( = 20), Group B ( = 20), and Group A specimens were stained with fluorescein diacetate dye for the detection of viability and adherence Group B were stained with acridine orange dye for detection of metabolic activity and adherence. Samples were washed, thoroughly sectioned and examined by confocal laser scanning microscopy. Computer-assisted determinants of fluorescence, bacterial viability, metabolic activity, and adherence were compared statistically.
was able to invade the dentinal tubules to a depth of 1-400 μm and adhere to 1-200 μm depth. Adherence (90%) was significantly higher in 1-100 μm using fluorescein diacetate and acridine orange dye.
Adherence of as evaluated by confocal laser scanning microscope was highest at the depth of 1-100 μm which may have an impact on the shaping and cleaning procedures on the root canal.
本研究旨在使用共聚焦激光扫描显微镜检测[细菌名称未明确]对人离体牙感染牙本质小管的黏附情况。
选取40颗人前磨牙牙根，在脑心浸液中用美国典型培养物保藏中心29212菌株感染21天。实验期结束后，标本分为两组，A组（20颗），B组（20颗），A组标本用二醋酸荧光素染料染色以检测活力和黏附情况，B组用吖啶橙染料染色以检测代谢活性和黏附情况。样本经冲洗、彻底切片后，用共聚焦激光扫描显微镜检查。对荧光、细菌活力、代谢活性和黏附情况的计算机辅助测定结果进行统计学比较。
[细菌名称未明确]能够侵入牙本质小管达1 - 400μm深度，并在1 - 200μm深度黏附。使用二醋酸荧光素和吖啶橙染料时，在1 - 100μm处的黏附率（90%）显著更高。
通过共聚焦激光扫描显微镜评估，[细菌名称未明确]在1 - 100μm深度的黏附率最高，这可能会对根管的成形和清洁程序产生影响。
