Nivinskas R, Vaiskunaite R, Raudonikiene A
Institute of Biochemistry, Lithuanian Academy of Sciences, Vilnius.
Mol Gen Genet. 1992 Mar;232(2):257-61. doi: 10.1007/BF00280004.
Bacteriophage T4 baseplate gene 26 codes for two in-frame overlapping peptides with identical C-terminal regions. By site-directed mutagenesis we have now determined that an internal AUU, codon 114 of gene 26, is used as the initiation codon for the synthesis of a smaller peptide (gp26*). Thus gene 26* gives rise to a peptide of 95 amino acid residues with an Mr of 10,873, while the complete gene 26 encodes a peptide of 208 amino acid residues of M(r) 23,880. Expression of gene 26* is shown to depend on the RNA secondary structure in the translational initiation region of this gene.
噬菌体T4基板基因26编码两个读框内重叠的肽段，它们具有相同的C末端区域。通过定点诱变，我们现已确定基因26的第114位密码子内部的AUU用作合成较小肽段（gp26*）的起始密码子。因此，基因26产生一个由95个氨基酸残基组成、Mr为10873的肽段，而完整的基因26编码一个由208个氨基酸残基组成、M(r)为23880的肽段。研究表明，基因26的表达取决于该基因翻译起始区域的RNA二级结构。
