Döll S, Dänicke S, Valenta H, Flachowsky G
Institute of Animal Nutrition, Federal Agricultural Research Centre (FAL), Braunschweig, Germany.
Arch Anim Nutr. 2004 Aug;58(4):311-24. doi: 10.1080/00039420412331273268.
A simple in vitro system was developed to study the efficacy of commercially available mycotoxin detoxifying agents and adsorbing substances as feed additives to detoxify deoxynivalenol (DON) and zearalenone (ZON) in situ. The in vitro model simulates the conditions (pH, temperature and transit time) of the porcine gastrointestinal tract, as pigs react most sensitively to these mycotoxins. The commercially available products were not effective in detoxifying DON and ZON under the applied conditions, while activated carbon was able to bind both toxins and cholestyramine, and a modified aluminosilicate showed good adsorption abilities for ZON. Data obtained in dose dependency studies showed an estimated adsorption capacity of cholestyramine and the modified aluminosilicate of 11.7 and 5.7 g ZON/kg detoxifying agent. The in vitro system deployed in the present study was demonstrated to be a simple, helpful tool in screening substances for their ability to detoxify DON and ZON under the simulated conditions of the porcine gastrointestinal tract. Nonetheless in vivo experiments are indispensable to proof the efficacy.
开发了一种简单的体外系统，以研究市售霉菌毒素解毒剂和吸附物质作为饲料添加剂原位解毒脱氧雪腐镰刀菌烯醇（DON）和玉米赤霉烯酮（ZON）的效果。该体外模型模拟了猪胃肠道的条件（pH值、温度和通过时间），因为猪对这些霉菌毒素反应最为敏感。在所应用的条件下，市售产品对DON和ZON解毒无效，而活性炭能够结合这两种毒素以及消胆胺，并且一种改性铝硅酸盐对ZON显示出良好的吸附能力。剂量依赖性研究获得的数据表明，消胆胺和改性铝硅酸盐对ZON的估计吸附容量分别为11.7和5.7 g ZON/kg解毒剂。本研究中使用的体外系统被证明是一种简单、有用的工具，可用于筛选物质在猪胃肠道模拟条件下解毒DON和ZON的能力。尽管如此，体内实验对于证明疗效是必不可少的。
