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[反相高效液相色谱法测定不同生长时期狗枣猕猴桃叶中丁香苷C含量的变化研究]

[Study on variation of actinoside C in leaves of Actinidia kolomikta with different growth periods by RP-HPLC].

作者信息

Jin Yong-Ri, Gui Ming-Yu, Lu Juan, Li Xu-Wen, Xu Ji-Qing

机构信息

Chemistry College of Jilin University, Changchun 130012, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Sep;32(18):1898-900.

PMID:18051901
Abstract

OBJECTIVE

To determine actinoside C in the leaves of Actinidia kolomikta with different growth periods.

METHOD

The separation was performed at 25 degrees C on ZORBAX Extend C18 column (4.6 mm x 250 mm, 5 microm), using amixture of methanol and water (51:49) as a mobile phase. The flow rate was 1.2 mL x min(-1), and the wavelength for measurement was 267 nm.

RESULT

The results showed that the contents of actinoside C in the leaves of A. kolomikta were variety in different growth periods. Actinoside C could reach its highest content in the middle ten days of June, then the content would decrease in the middle ten days of July slightly, it could reach their lowest content in the middle ten days of August.

CONCLUSION

The optimal collective date for A. kolomikta are in the middle ten days of June.

摘要

目的

测定不同生长时期狗枣猕猴桃叶片中紫丁香苷C的含量。

方法

采用ZORBAX Extend C18柱(4.6 mm×250 mm,5μm),在25℃下进行分离,以甲醇和水(51:49)的混合溶液为流动相。流速为1.2 mL·min⁻¹,测定波长为267 nm。

结果

结果表明,狗枣猕猴桃叶片中紫丁香苷C的含量在不同生长时期有所不同。紫丁香苷C在6月中旬含量最高,7月中旬含量略有下降,8月中旬含量最低。

结论

狗枣猕猴桃的最佳采收期为6月中旬。

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