Hamada Atsuhide, Yoshioka Saburo, Takuma Daisuke, Yokota Junko, Cui Tailine, Kusunose Masahiko, Miyamura Mitsuhiko, Kyotani Shojiro, Nishioka Yutaka
Department of Pharmacy, Kochi Medical School Hospital, Japan.
Biol Pharm Bull. 2004 Dec;27(12):1961-4. doi: 10.1248/bpb.27.1961.
Eriobotrya japonica has been used as a medicinal plant for a long time, and its leaves are known to have many physiological actions such as anti-inflammatory, antitussive, and expectoran. In contrast, Eriobotrya japonica seeds are only known to contain amygdalin, and almost no investigations of its pharmacological action have been performed. Moreover, some anticancer agents such as adriamycin cause renal disorders as an adverse effect, and the mechanism of the adverse effect is considered to involve oxidative stress. We have reported that Eriobotrya japonica seed extract has an inhibitory effect on liver disorders. In this study, we prepared a 70% ethanol extract of Eriobotrya japonica seeds and administered the extract to rats with renal disorder induced by a single administration of 7 mg/kg body weight adriamycin, and investigated the usefulness of the extract. Increases in indices of renal function, plasma urea nitrogen, were significantly inhibited in rats treated with the Eriobotrya japonica extract compared to rats treated with tap water. In addition, the renal tissue level of reduced glutathione was significantly high in rats that ingested the extract, while the lipid peroxide levels in plasma and renal tissue were significantly low. However, no effect on renal tissue antioxidative enzymes was observed, suggesting that Eriobotrya japonica seed extract has direct antioxidative action. Based on these findings, Eriobotrya japonica seed extract may be effective in reducing the oxidative stress of adriamycin-induced renal disorder. Therefore, ingestion of Eriobotrya japonica seed extract may contribute to a reduction of the adverse effects of adriamycin.
枇杷长期以来一直被用作药用植物，其叶子具有多种生理作用，如抗炎、镇咳和祛痰作用。相比之下，枇杷种子仅已知含有苦杏仁苷，几乎没有对其药理作用进行过研究。此外，一些抗癌药物如阿霉素会导致肾脏疾病作为副作用，并且认为副作用的机制涉及氧化应激。我们已经报道枇杷种子提取物对肝脏疾病有抑制作用。在本研究中，我们制备了枇杷种子的70%乙醇提取物，并将其给予单次腹腔注射7mg/kg体重阿霉素诱导的肾脏疾病大鼠，研究该提取物的有效性。与给予自来水的大鼠相比，用枇杷提取物处理的大鼠肾功能指标血浆尿素氮的升高受到显著抑制。此外，摄入提取物的大鼠肾脏组织中还原型谷胱甘肽水平显著升高，而血浆和肾脏组织中的脂质过氧化物水平显著降低。然而，未观察到对肾脏组织抗氧化酶的影响，表明枇杷种子提取物具有直接的抗氧化作用。基于这些发现，枇杷种子提取物可能对减轻阿霉素诱导的肾脏疾病的氧化应激有效。因此，摄入枇杷种子提取物可能有助于减少阿霉素的副作用。
