Department of Pharmacy, Kochi Medical School Hospital, Kohasu, Oko-cho, Nankoku, Kochi 783-8505, Japan.
J Nat Med. 2011 Jan;65(1):9-17. doi: 10.1007/s11418-010-0445-2. Epub 2010 Jul 17.
We have previously reported that Eriobotrya japonica seed extract (ESE) is effective for the treatment of various gastric mucosal injuries. For the pharmaceutical preparation of ESE, we are evaluating deep sea water (DSW), which contains trace elements and has a homeostasis-enhancing effect, as the solvent. In this study, we prepared DSW containing ESE (ESE + DSW) and evaluated its usefulness for the prevention of gastric mucosal injuries using non-steroidal anti-inflammatory drug-induced acute gastric mucosal injury models in male Wistar/ST rats. Gastric mucosal injury models were prepared by administering indomethacin at 30 mg/kg orally to the rats after a 24-h fast. ESE was prepared by a routine procedure and administered at the same concentration as in the administration to humans. The rats were divided into the following 6 groups: ESE, DSW, ESE + DSW, tap water (control), rebamipide (positive control), or untreated. Gastric mucosal injuries were evaluated by measuring the injury area, lipid peroxide (LPO) level, antioxidative enzyme level, and volume of mucus. The injury area and LPO levels in plasma and gastric tissue were significantly reduced in the ESE and ESE + DSW groups compared with the control and DSW group. The plasma and gastric tissue antioxidative enzyme levels were significantly higher in the ESE and ESE + DSW groups than in the control group. These results suggest that DSW, when combined with ESE, inhibits antioxidative enzymes, and enhances the gastric mucosal protecting effect of ESE.
我们之前曾报道过，枇杷种子提取物（ESE）对治疗各种胃黏膜损伤有效。对于 ESE 的药物制剂，我们正在评估含有微量元素且具有增强内稳态作用的深海水（DSW）作为溶剂。在这项研究中，我们制备了含有 ESE 的 DSW（ESE+DSW），并使用非甾体抗炎药诱导的雄性 Wistar/ST 大鼠急性胃黏膜损伤模型评估其预防胃黏膜损伤的用途。胃黏膜损伤模型通过在禁食 24 小时后给大鼠口服吲哚美辛 30mg/kg 来制备。ESE 按照常规程序制备，并以与给人类相同的浓度给予。大鼠分为以下 6 组：ESE、DSW、ESE+DSW、自来水（对照）、瑞巴派特（阳性对照）或未处理。通过测量损伤面积、脂质过氧化物（LPO）水平、抗氧化酶水平和粘液量来评估胃黏膜损伤。与对照组和 DSW 组相比，ESE 和 ESE+DSW 组的损伤面积和血浆及胃组织中的 LPO 水平显著降低。ESE 和 ESE+DSW 组的血浆和胃组织抗氧化酶水平明显高于对照组。这些结果表明，DSW 与 ESE 结合可抑制抗氧化酶，增强 ESE 的胃黏膜保护作用。
