单链DNA中4-硝基喹啉1-氧化物的突变谱以鸟嘌呤到嘧啶的颠换为特征。
The 4-nitroquinoline 1-oxide mutational spectrum in single stranded DNA is characterized by guanine to pyrimidine transversions.
作者信息
Fronza G, Campomenosi P, Iannone R, Abbondandolo A
机构信息
Laboratory of Mutagenesis, National Institute for Research on Cancer (IST), Genova, Italy.
出版信息
Nucleic Acids Res. 1992 Mar 25;20(6):1283-7. doi: 10.1093/nar/20.6.1283.
Abstract
4-Nitroquinoline-1-oxide is a potent mutagen and carcinogen which induces two main guanine adducts at positions C8 and N2. In ds or ss damaged DNA the ratio C8/N2 adducts is 1:2 and 8-10:1, respectively. In bacteria and yeast 4NQO has been shown to be a base substitution mutagen acting at G residues inducing mainly G to A transitions. We determined the mutational spectrum induced by the 4NQO metabolite, acetoxy-4-aminoquinoline 1-oxide, in the M13lacZ'/E. coli lacZ delta M15 alpha complementation assay using ssDNA. Among 68 Ac-4HAQO induced mutants, G to Pyr transversion was the most frequent base substitution observed. By comparison with dsDNA based systems, our data suggest that dGuo-C8-AQO induces G to Pyr transversions. A mechanism to explain how this lesion may induce transversions is proposed.
摘要
4-硝基喹啉-1-氧化物是一种强效诱变剂和致癌物，它会在鸟嘌呤的C8和N2位置诱导产生两种主要的加合物。在双链或单链受损DNA中，C8/N2加合物的比例分别为1:2和8 - 10:1。在细菌和酵母中，4NQO已被证明是一种碱基置换诱变剂，作用于鸟嘌呤残基，主要诱导G到A的转换。我们在使用单链DNA的M13lacZ'/大肠杆菌lacZ delta M15α互补试验中，测定了4NQO代谢物乙酰氧基-4-氨基喹啉1-氧化物诱导的突变谱。在68个由Ac - 4HAQO诱导的突变体中，G到嘧啶的颠换是观察到的最常见的碱基置换。通过与基于双链DNA的系统进行比较，我们的数据表明dGuo - C8 - AQO会诱导G到嘧啶的颠换。本文提出了一种机制来解释这种损伤如何诱导颠换。
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本文引用的文献
1
Sensitivity of the conformation of deoxyguanosine to binding at the C-8 position by N-acetylated and unacetylated 2-aminofluorene.脱氧鸟苷构象对N - 乙酰化和未乙酰化的2 - 氨基芴在C - 8位结合的敏感性。
Carcinogenesis. 1980;1(11):955-9. doi: 10.1093/carcin/1.11.955.
2
In vitro DNA reaction with a carcinogen: the O,O'-diacetyl-4-hydroxyaminoquinoline 1-oxide changes of stability of modified DNA.致癌物与体外DNA的反应：O，O'-二乙酰-4-羟基氨基喹啉1-氧化物对修饰DNA稳定性的影响
Biochim Biophys Acta. 1980 Oct 17;609(3):383-91. doi: 10.1016/0005-2787(80)90112-4.
3
A transversion mutation hypothesis for chemical carcinogenesis by N2-substitution of guanine in DNA.关于DNA中鸟嘌呤N2位取代导致化学致癌作用的颠换突变假说。
Chem Biol Interact. 1980 Sep;31(3):255-63. doi: 10.1016/0009-2797(80)90014-9.
4
5
Hot spots of frameshift mutations induced by the ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene.由终极致癌物N-乙酰氧基-N-2-乙酰氨基芴诱导的移码突变热点。
Nature. 1981 Dec 17;294(5842):657-9. doi: 10.1038/294657a0.
6
Identification of 4-acetoxyaminoquinoline from the hydrolysis of 1-acetoxy-4-acetoxyimino-1,4-dihydroquinoline, in vitro and in vivo properties.从1-乙酰氧基-4-乙酰氧基亚氨基-1,4-二氢喹啉水解产物中鉴定4-乙酰氧基氨基喹啉及其体外和体内性质。
Chem Biol Interact. 1983 Jan;43(1):87-98. doi: 10.1016/0009-2797(83)90106-0.
7
Sequence analysis of ultraviolet-induced mutations in M13lacZ hybrid phage DNA.M13lacZ杂交噬菌体DNA中紫外线诱导突变的序列分析。
J Mol Biol. 1984 Dec 5;180(2):217-37. doi: 10.1016/s0022-2836(84)80001-7.
8
Guanyl-C8-arylamination of DNA by the ultimate carcinogen of 4-nitroquinoline-1-oxide: a spectrophotometric titration.4-硝基喹啉-1-氧化物的最终致癌物对DNA的鸟嘌呤-C8-芳基胺化作用：分光光度滴定法
Anal Biochem. 1984 May 1;138(2):454-7. doi: 10.1016/0003-2697(84)90839-x.
9
Mutational specificity of depurination.脱嘌呤的突变特异性
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1494-8. doi: 10.1073/pnas.81.5.1494.
10
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
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