Jacquier A, Legrain P, Dujon B
Unité de Génétique Moléculaire des Levures (URA 1149 du CNRS), Institut Pasteur, Paris, France.
Yeast. 1992 Feb;8(2):121-32. doi: 10.1002/yea.320080207.
We report here the DNA sequence of a segment of chromosome XI of Saccharomyces cerevisiae extending over 10.7 kb. The sequence was determined using a double-strand sequencing strategy adapted from the random-clone strategy. The segment contains seven non-overlapping long open reading frames, YKL500, 505, 510, 513, 516, 518 and 520 and one tRNA gene. YKL505 and YKL513 are two already sequenced genes, the BAF1/ABF1 and the APN1 genes, respectively. YKL510 exhibits a strong homology to the RAD2 protein and YKL516 is presumably a protein kinase.
我们在此报告酿酒酵母第十一条染色体上一段长度超过10.7 kb的DNA序列。该序列是采用一种基于随机克隆策略改编的双链测序策略测定得到的。这段序列包含7个不重叠的长开放阅读框,即YKL500、505、510、513、516、518和520以及1个tRNA基因。YKL505和YKL513分别是两个已测序的基因,即BAF1/ABF1基因和APN1基因。YKL510与RAD2蛋白具有高度同源性,而YKL516推测是一种蛋白激酶。