Li Yang-jun, Yang Xin-guang, Gao Mei-rui
Department of Ophthalmology, Tang Du Hospital, The Fourth Military Medical University, Xi'An 710038, Shanxi, China.
Zhongguo Zhong Yao Za Zhi. 2003 Oct;28(10):961-4.
To study the effects of extracts of ginkgo biloba (EGB) on the levels of nitric oxide (NO) and apoptosis in the retina induced by glutamate by intravitreal injection in adult rabbit.
The model of apoptosis in retina induced by glutamate by intravitreal injection was established in adult rabbit and EGB was retrobulbarly injected. The levels of NO were measured by spectrophotometer. Retina DNA fragmentation was determined by agarose gel electrophoresis.
The levels of NO in retina in experiment groups were significantly increased compared with controls, after treatment with high dosage of EGB, and levels of NO was decreased to normal. They were not decreased by injection of small dosage of EGB. DNA fragmentation of retina apoptosis was detected in experiment groups and small dosage EGB groups.
The retina apoptosis was induced by glutamate by intravitreal injection in adult rabbit and was inhibited by EGB that may be through blocking the generation of NO free radicals.
通过成年兔玻璃体内注射，研究银杏叶提取物（EGB）对谷氨酸诱导的视网膜中一氧化氮（NO）水平及细胞凋亡的影响。
成年兔建立玻璃体内注射谷氨酸诱导视网膜细胞凋亡的模型，并球后注射EGB。用分光光度计测量NO水平。通过琼脂糖凝胶电泳测定视网膜DNA片段化。
与对照组相比，实验组视网膜中NO水平显著升高，高剂量EGB治疗后，NO水平降至正常。小剂量EGB注射未使其降低。实验组和小剂量EGB组均检测到视网膜凋亡的DNA片段化。
成年兔玻璃体内注射谷氨酸可诱导视网膜细胞凋亡，EGB可能通过阻断NO自由基的产生而抑制这种凋亡。
