Zahr Alisar S, de Villiers Melgardt, Pishko Michael V
Departments of Chemical Engineering, Chemistry, and Materials Science and Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
Langmuir. 2005 Jan 4;21(1):403-10. doi: 10.1021/la0478595.
Layer-by-Layer (LbL) stepwise self-assembly of the polyelectrolytes poly(allylamine hydrochloride) and poly(styrenesulfonate) was used to create a macromolecular nanoshell around drug nanoparticles (approximately 150 nm in diameter). Dexamethasone, a steroid often used in conjugation with chemotherapy, was chosen as a model drug and was formulated into nanoparticles using a modified solvent-evaporation emulsification method. Measurement of the zeta potential (zeta-potential) after each polyelectrolyte layer was electrostatically added confirmed the successful addition of each layer. Additionally, data acquired from X-ray photon spectroscopy (XPS) indicated the presence of peaks representative of each physisorbed polyelectrolyte layer. Surface modification of the nanoshell was performed by covalently attaching poly(ethylene glycol) (PEG) with a molecular weight of 2000 to the outer surface of the nanoshell. Zeta potential measurements and XPS indicated the presence of PEG chains at the surface of the nanoshell. The polymeric nanoshell on the surface of the drug nanoparticle provides a template upon which surface modifications can be made to create a stealth or targeted drug delivery system.
采用聚（烯丙胺盐酸盐）和聚（苯乙烯磺酸盐）这两种聚电解质的逐层（LbL）逐步自组装方法，在药物纳米颗粒（直径约150 nm）周围构建了一个大分子纳米壳。地塞米松是一种常用于与化疗联合使用的类固醇，被选为模型药物，并采用改良的溶剂蒸发乳化法将其制成纳米颗粒。在静电添加每一层聚电解质后测量zeta电位（ζ电位），证实了每一层的成功添加。此外，从X射线光子能谱（XPS）获得的数据表明存在代表每个物理吸附聚电解质层的峰。通过将分子量为2000的聚（乙二醇）（PEG）共价连接到纳米壳的外表面，对纳米壳进行了表面修饰。zeta电位测量和XPS表明纳米壳表面存在PEG链。药物纳米颗粒表面的聚合物纳米壳提供了一个模板，可在其上进行表面修饰以创建隐形或靶向药物递送系统。
