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公猪精液中的病毒:人工授精传播疾病的检测、临床及流行病学后果

Viruses in boar semen: detection and clinical as well as epidemiological consequences regarding disease transmission by artificial insemination.

作者信息

Guérin B, Pozzi N

机构信息

Laboratoire National pour le Contrôle des Reproducteurs, 94703 Maisons-Alfort, France.

出版信息

Theriogenology. 2005 Jan 15;63(2):556-72. doi: 10.1016/j.theriogenology.2004.09.030.

Abstract

Many viruses have been reported to be present in boar semen, particularly during the viremic phase of the diseases. Some of them, such foot-and-mouth disease virus, porcine reproductive and respiratory syndrome virus, swine vesicular disease virus, porcine parvovirus, picornaviruses, adenoviruses, enteroviruses, Japanese encephalitis virus, pseudorabies virus, African swine fever virus and reoviruses are of particular importance and accurate monitoring prior to and during the presence of boars in AI stations is essential. Various methods may be used to detect these viruses in the animals, or even directly in batches of semen. Cell culture, ELISA and PCR are the most accurate and widely used. Because of the high risk of dissemination of disease via AI, the absolute goal is to provide pathogen-free semen and this is feasible with the adequate measures that are discussed briefly in this paper.

摘要

据报道,公猪精液中存在多种病毒,尤其是在疾病的病毒血症阶段。其中一些病毒,如口蹄疫病毒、猪繁殖与呼吸综合征病毒、猪水疱病病毒、猪细小病毒、小RNA病毒、腺病毒、肠道病毒、日本脑炎病毒、伪狂犬病病毒、非洲猪瘟病毒和呼肠孤病毒,尤为重要,在人工授精站公猪进站前和进站期间进行准确监测至关重要。可采用多种方法检测动物体内甚至直接检测批次精液中的这些病毒。细胞培养、酶联免疫吸附测定和聚合酶链反应是最准确且应用最广泛的方法。由于通过人工授精传播疾病的风险很高,绝对目标是提供无病原体的精液,而通过本文简要讨论的适当措施这是可行的。

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