al-Khodairy F, Carr A M
MRC Cell Mutation Unit, Sussex University, Falmer, UK.
EMBO J. 1992 Apr;11(4):1343-50. doi: 10.1002/j.1460-2075.1992.tb05179.x.
We have tested mutants corresponding to 20 DNA repair genes of the fission yeast Schizosaccharomyces pombe for their ability to arrest in G2 after DNA damage. Of the mutants tested, four are profoundly defective in this damage dependent G2 arrest. In addition, these four mutants are highly sensitive to a transient inhibition of DNA synthesis by hydroxyurea. This suggests that the pathway responsible for the recognition of DNA damage and the subsequent mitotic arrest, shares many functions with the mechanism that controls the dependency of mitosis on the completion of S phase. The phenotype of these checkpoint rad mutants in wee mutant backgrounds indicate that the G2 arrest response is mediated either through, or in parallel with, the activity of the cdc2 gene product.
我们已经测试了粟酒裂殖酵母20个DNA修复基因对应的突变体在DNA损伤后停滞在G2期的能力。在所测试的突变体中，有四个在这种依赖损伤的G2期停滞方面存在严重缺陷。此外，这四个突变体对羟基脲对DNA合成的短暂抑制高度敏感。这表明负责识别DNA损伤及随后有丝分裂停滞的途径，与控制有丝分裂依赖于S期完成的机制具有许多共同功能。这些检查点rad突变体在wee突变体背景下的表型表明，G2期停滞反应是通过cdc2基因产物的活性介导的，或者与之平行。
