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High prevalence of respiratory viral infections in patients hospitalized in an intensive care unit for acute respiratory infections as detected by nucleic acid-based assays.通过核酸检测法发现，在因急性呼吸道感染入住重症监护病房的患者中，呼吸道病毒感染的患病率很高。
J Clin Microbiol. 2005 Jan;43(1):455-7. doi: 10.1128/JCM.43.1.455-457.2005.
2
Respiratory viruses in bronchoalveolar lavage: a hospital-based cohort study in adults.支气管肺泡灌洗中的呼吸道病毒：一项基于医院的成人队列研究
Thorax. 2009 May;64(5):399-404. doi: 10.1136/thx.2008.105155. Epub 2009 Jan 27.
3
Lower respiratory tract viral infections in hospitalized adult patients.住院成人患者下呼吸道病毒感染。
Minerva Med. 2009 Oct;100(5):349-55.
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Frequency of detection of respiratory viruses in the lower respiratory tract of hospitalized adults.住院成人下呼吸道中呼吸道病毒的检测频率
J Clin Virol. 2008 Jun;42(2):215-20. doi: 10.1016/j.jcv.2008.01.012. Epub 2008 Mar 14.
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Detection of human herpesvirus-7 DNA in bronchoalveolar lavage.检测支气管肺泡灌洗液中人疱疹病毒 7 型 DNA。
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Viral pathogens associated with acute respiratory infections in central vietnamese children.与越南北中部儿童急性呼吸道感染相关的病毒病原体。
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Frequent detection of viral coinfection in children hospitalized with acute respiratory tract infection using a real-time polymerase chain reaction.采用实时聚合酶链反应对因急性呼吸道感染住院的儿童进行病毒合并感染的频繁检测。
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Respiratory viral coinfections identified by a 10-plex real-time reverse-transcription polymerase chain reaction assay in patients hospitalized with severe acute respiratory illness--South Africa, 2009-2010.10 重实时逆转录聚合酶链反应检测鉴定的住院严重急性呼吸道疾病患者中的呼吸道病毒合并感染 - 南非，2009-2010 年。
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Lower respiratory viral illnesses: improved diagnosis by molecular methods and clinical impact.下呼吸道病毒性疾病：分子方法改善诊断及临床影响
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Simultaneous detection of different respiratory virus by a multiplex reverse transcription polymerase chain reaction combined with flow-through reverse dot blotting assay.通过多重逆转录聚合酶链反应结合流通式反向斑点杂交法同时检测不同呼吸道病毒。
Diagn Microbiol Infect Dis. 2008 Sep;62(1):44-51. doi: 10.1016/j.diagmicrobio.2008.04.017. Epub 2008 Jul 18.

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Epidemiologic Characteristics and Clinical Significance of Respiratory Viral Infections Among Adult Patients Admitted to the Intensive Care Unit.入住重症监护病房的成年患者呼吸道病毒感染的流行病学特征及临床意义
Front Med (Lausanne). 2022 May 24;9:829624. doi: 10.3389/fmed.2022.829624. eCollection 2022.
2
[Current techniques used for the diagnosis of respiratory virus infectious in intensive care units].[重症监护病房中用于诊断呼吸道病毒感染的当前技术]
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[Ventilator-associated viral pneumonia].呼吸机相关性病毒性肺炎
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Viral Pneumonia and Acute Respiratory Distress Syndrome.病毒性肺炎与急性呼吸窘迫综合征
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Burden and viral aetiology of influenza-like illness and acute respiratory infection in intensive care units.重症监护病房中流感样疾病和急性呼吸道感染的负担及病毒病因
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Burden of respiratory viruses in patients with acute respiratory failure.急性呼吸衰竭患者的呼吸道病毒负担。
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Respiratory virology and microbiology in intensive care units: a prospective cohort study.重症监护病房的呼吸病毒学和微生物学：一项前瞻性队列研究。
APMIS. 2013 Nov;121(11):1097-108. doi: 10.1111/apm.12089. Epub 2013 May 18.
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[Acute viral infections in immunocompetent patients].[免疫功能正常患者的急性病毒感染]
Med Intensiva. 2011 Apr;35(3):179-85. doi: 10.1016/j.medin.2011.01.001. Epub 2011 Feb 24.
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Detection of respiratory viruses and the associated chemokine responses in serious acute respiratory illness.严重急性呼吸道疾病中呼吸道病毒的检测及相关趋化因子反应。
Thorax. 2010 Jul;65(7):639-44. doi: 10.1136/thx.2009.132480.

本文引用的文献

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Viral community-acquired pneumonia in nonimmunocompromised adults.非免疫功能低下成人的病毒性社区获得性肺炎
Chest. 2004 Apr;125(4):1343-51. doi: 10.1378/chest.125.4.1343.
2
Airway colonisation in long-term mechanically ventilated patients. Effect of semi-recumbent position and continuous subglottic suctioning.长期机械通气患者的气道定植。半卧位和持续声门下吸引的效果。
Intensive Care Med. 2004 Feb;30(2):225-233. doi: 10.1007/s00134-003-2077-4. Epub 2003 Nov 29.
3
Clinical potential of the acyclic nucleoside phosphonates cidofovir, adefovir, and tenofovir in treatment of DNA virus and retrovirus infections.无环核苷膦酸酯西多福韦、阿德福韦和替诺福韦在治疗DNA病毒和逆转录病毒感染方面的临床潜力。
Clin Microbiol Rev. 2003 Oct;16(4):569-96. doi: 10.1128/CMR.16.4.569-596.2003.
4
Effectiveness of neuraminidase inhibitors in treatment and prevention of influenza A and B: systematic review and meta-analyses of randomised controlled trials.神经氨酸酶抑制剂治疗和预防甲型与乙型流感的有效性：随机对照试验的系统评价与荟萃分析
BMJ. 2003 Jun 7;326(7401):1235. doi: 10.1136/bmj.326.7401.1235.
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Virological features and clinical manifestations associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratory-tract infections in all age groups.与人类偏肺病毒相关的病毒学特征及临床表现：一种可导致各年龄组急性呼吸道感染的新型副粘病毒
J Infect Dis. 2002 Nov 1;186(9):1330-4. doi: 10.1086/344319. Epub 2002 Oct 8.
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Severe community-acquired pneumonia.重症社区获得性肺炎
Curr Opin Crit Care. 2002 Oct;8(5):453-60. doi: 10.1097/00075198-200210000-00014.
7
Severe community-acquired pneumonia: current outcomes, epidemiology, etiology, and therapy.重症社区获得性肺炎：当前的治疗结果、流行病学、病因及治疗方法
Curr Opin Infect Dis. 2001 Dec;14(6):703-9. doi: 10.1097/00001432-200112000-00007.
8
Polymerase chain reaction is more sensitive than viral culture and antigen testing for the detection of respiratory viruses in adults with hematological cancer and pneumonia.对于检测患有血液系统癌症和肺炎的成人呼吸道病毒，聚合酶链反应比病毒培养和抗原检测更敏感。
Clin Infect Dis. 2002 Jan 15;34(2):177-83. doi: 10.1086/338238. Epub 2001 Dec 4.
9
Respiratory virus infections after stem cell transplantation: a prospective study from the Infectious Diseases Working Party of the European Group for Blood and Marrow Transplantation.造血干细胞移植后的呼吸道病毒感染：欧洲血液与骨髓移植组传染病工作组的一项前瞻性研究
Bone Marrow Transplant. 2001 Sep;28(5):479-84. doi: 10.1038/sj.bmt.1703139.
10
Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction.通过聚合酶链反应直接诊断人类呼吸道冠状病毒229E和OC43
J Virol Methods. 2001 Sep;97(1-2):59-66. doi: 10.1016/s0166-0934(01)00343-3.

通过核酸检测法发现，在因急性呼吸道感染入住重症监护病房的患者中，呼吸道病毒感染的患病率很高。

High prevalence of respiratory viral infections in patients hospitalized in an intensive care unit for acute respiratory infections as detected by nucleic acid-based assays.

作者信息

Legoff Jérôme, Guérot Emmanuel, Ndjoyi-Mbiguino Angélique, Matta Mathieu, Si-Mohamed Ali, Gutmann Laurent, Fagon Jean-Yves, Bélec Laurent

机构信息

Hôpital Européen Georges Pompidou, and Unité INSERM 430, Institut de Recherches Biomédicales des Cordeliers, Faculté de Médecine Broussais-Hôtel Dieu, Université Pierre et Marie Curie, Paris, France.

出版信息

J Clin Microbiol. 2005 Jan;43(1):455-7. doi: 10.1128/JCM.43.1.455-457.2005.

DOI:10.1128/JCM.43.1.455-457.2005
PMID:15635014
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC540110/
Abstract

Forty-seven bronchoalveolar lavages (BAL) were obtained from 41 patients with acute pneumonia attending an intensive care unit. By molecular diagnosis, 30% of total BAL and 63% of bacteria-negative BAL were positive for respiratory viruses. Molecular detection allows for high-rate detection of respiratory viral infections in adult patients suffering from severe pneumonia.

摘要

从入住重症监护病房的41例急性肺炎患者中获取了47份支气管肺泡灌洗样本（BAL）。通过分子诊断，在全部BAL样本中30%以及细菌阴性的BAL样本中63%检测出呼吸道病毒呈阳性。分子检测能够高效检测出患有严重肺炎的成年患者中的呼吸道病毒感染。