Hari Kumar K B, Sabu M C, Lima P S, Kuttan Ramadasan
Amala Cancer Research Centre, Thrissur, Kerala State, India.
J Radiat Res. 2004 Dec;45(4):549-55. doi: 10.1269/jrr.45.549.
The radio protective effect of the fruit pulp of Emblica officinalis Gaertn (Emblica) was studied in adult Swiss albino mice. Mice were treated with 2.5 g/kg b.wt of Emblica for 10 consecutive days before irradiation and exposed to a single dose of 700 rads (7Gy) of radiation after the last dose. One group was given Emblica continuously for another 15 days after irradiation. Changes in the total leukocyte count, bone marrow viability and hemoglobin were studied after whole body irradiation. Administration of Emblica significantly increased these levels, which were lowered by irradiation. Animals were sacrificed at various time points after irradiation and the activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), and glutathione-S-transferase (GST), and levels of glutathione were assayed in the blood. The damage to the cell membrane after whole body irradiation was studied by measuring the tissue lipid peroxides levels. Administration of Emblica significantly enhanced the activity of the various antioxidant enzymes and GST as well as glutathione system in the blood. Treatment with Emblica also lowered the elevated levels of lipid peroxides in the serum. The data clearly indicated that the extract significantly reduced the bioeffects of radiation. Emblica extract may be useful in reducing the side effects produced during therapeutic radiation.
在成年瑞士白化小鼠中研究了余甘子（Emblica officinalis Gaertn）果肉的辐射防护作用。在照射前，小鼠连续10天接受2.5 g/kg体重的余甘子处理，在最后一剂后接受单次700拉德（7Gy）的辐射。一组在照射后再连续给予余甘子15天。在全身照射后研究了白细胞总数、骨髓活力和血红蛋白的变化。给予余甘子显著提高了这些因照射而降低的水平。在照射后的不同时间点处死动物，并测定血液中抗氧化酶过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）和谷胱甘肽-S-转移酶（GST）的活性以及谷胱甘肽水平。通过测量组织脂质过氧化物水平研究全身照射后对细胞膜的损伤。给予余甘子显著增强了血液中各种抗氧化酶、GST以及谷胱甘肽系统的活性。用余甘子处理还降低了血清中升高的脂质过氧化物水平。数据清楚地表明，该提取物显著降低了辐射的生物效应。余甘子提取物可能有助于减少治疗性辐射期间产生的副作用。
