Grasemann H, Grasemann C, Kurtz F, Tietze-Schillings G, Vester U, Ratjen F
Children's Hospital, University of Essen, Hufeland Str. 55, D-45122 Essen, Germany.
Eur Respir J. 2005 Jan;25(1):62-8. doi: 10.1183/09031936.04.00086104.
Exhaled nitric oxide (eNO) is decreased in cystic fibrosis (CF). The effect of oral L-arginine, the precursor of enzymatic nitric oxide (NO) formation, on airway NO in patients with CF was studied. In a pilot study, oral L-arginine was given in a single dose of 200 mg x kg(-1) body weight to eight healthy controls and eight CF patients. Subsequently, the same L-arginine dose was given to 10 patients with CF (five females) t.i.d. for 6 weeks in a randomised double-blind placebo-controlled crossover study. A single dose of oral L-arginine resulted in a 5.5-fold increase of L-arginine in plasma and a 1.3-fold increase of L-arginine in sputum after 2 h. Maximum eNO, within 3 h of L-arginine intake, increased significantly in both CF patients (5.4+/-2.1 ppb versus 8.3+/-3.5 ppb) and controls (18.0+/-8.1 ppb versus 26.4+/-12.3 ppb). Supplementation of L-arginine for 6 weeks resulted in a sustained increase in eNO compared to placebo (9.7+/-5.7 ppb versus 6.3+/-3.1 ppb). An effect of L-arginine supplementation on forced expiratory volume in one second was not observed. These data indicate that airway nitric oxide formation in cystic fibrosis patients can be augmented with oral L-arginine supplementation.
呼出一氧化氮（eNO）在囊性纤维化（CF）患者中减少。本研究探讨了一氧化氮（NO）酶促生成的前体——口服L-精氨酸对CF患者气道NO的影响。在一项初步研究中，给8名健康对照者和8名CF患者单次口服200 mg·kg⁻¹体重的L-精氨酸。随后，在一项随机双盲安慰剂对照交叉研究中，给10名CF患者（5名女性）每日3次给予相同剂量的L-精氨酸，持续6周。单次口服L-精氨酸2小时后，血浆中L-精氨酸增加5.5倍，痰液中L-精氨酸增加1.3倍。在摄入L-精氨酸后3小时内，CF患者（5.4±2.1 ppb对8.3±3.5 ppb）和对照者（18.0±8.1 ppb对26.4±12.3 ppb）的最大eNO均显著增加。与安慰剂相比，补充L-精氨酸6周导致eNO持续增加（9.7±5.7 ppb对6.3±3.1 ppb）。未观察到补充L-精氨酸对一秒用力呼气容积的影响。这些数据表明，口服补充L-精氨酸可增强囊性纤维化患者气道一氧化氮的生成。
