Li Wen-Jun, Chen Hua-Hong, Kim Chang-Jin, Zhang Yu-Qin, Park Dong-Jin, Lee Jae-Chan, Xu Li-Hua, Jiang Cheng-Lin
The Key Laboratory for Microbial Resources of Ministry of Education, PR China, Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Department of Chemistry, Chuxiong Normal College, Chuxiong, Yunnan 675000, PR China.
Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):463-466. doi: 10.1099/ijs.0.63281-0.
Two novel actinobacteria isolates, designated YIM 70009T and YIM 70081T, were characterized in order to determine their taxonomic position. Cells of strains YIM 70009T and YIM 70081T were cocci, although only the latter were motile. The G+C contents of their DNAs were 64.0 and 64.5 mol%, respectively. On the basis of chemotaxonomic characteristics and 16S rRNA gene sequence analysis, the two isolates were classified in the genus Nesterenkonia. DNA-DNA hybridization and comparison of phenotypic characteristics revealed that strains YIM 70009T and YIM 70081T differed from each other and from known species. Therefore, it is proposed that they represent two separate novel species of the genus Nesterenkonia: Nesterenkonia sandarakina sp. nov. (type strain, YIM 70009T=CCTCC AA 203007T=DSM 15664T=KCTC 19011T) and Nesterenkonia lutea sp. nov. (type strain, YIM 70081T=CCTCC AA 203010T=DSM 15666T=KCTC 19013T).
对两株新的放线菌分离株YIM 70009T和YIM 70081T进行了特征分析,以确定它们的分类地位。菌株YIM 70009T和YIM 70081T的细胞为球菌,不过只有后者具有运动性。它们DNA的G+C含量分别为64.0和64.5 mol%。基于化学分类特征和16S rRNA基因序列分析,这两株分离株被归入涅斯捷连科氏菌属。DNA-DNA杂交和表型特征比较表明,菌株YIM 70009T和YIM 70081T彼此不同,且与已知物种也不同。因此,建议将它们归为涅斯捷连科氏菌属的两个独立新物种:新种桑达腊涅斯捷连科氏菌(Nesterenkonia sandarakina sp. nov.)(模式菌株,YIM 70009T = CCTCC AA 203007T = DSM 15664T = KCTC 19011T)和新种黄色涅斯捷连科氏菌(Nesterenkonia lutea sp. nov.)(模式菌株,YIM 70081T = CCTCC AA 203010T = DSM 15666T = KCTC 19013T)。