NMPA Key Laboratory for Testing Technology of Pharmaceutical Microbiology, Shanghai Institute for Food and Drug Control, Shanghai, People's Republic of China.
Shaanxi Zhuzhijian Planning and Design Group Co., LTD, Xi'an, People's Republic of China.
Antonie Van Leeuwenhoek. 2024 Jan 3;117(1):8. doi: 10.1007/s10482-023-01905-1.
During our studies on the microorganism diversity from air of manufacturing shop in a pharmaceutical factory in Shandong province, China, a Gram-stain-positive, aerobic, cocci-shaped bacterium, designated LY-0111, was isolated from a settling dish. Strain LY-0111 grew at temperature of 10-42 °C (optimum 35 °C), pH of 5.0-10.0 (optimum pH 7.0) and NaCl concentration of 1-12% (optimum 0.5-3%, w/v). Based on the 16S rRNA gene sequence analysis, the strain shared the highest sequence similarities to Nesterenkonia halophila YIM 70179 (96.2%), and was placed within the radiation of Nesterenkonia species in the phylogenetic trees. The genome of the isolate was sequenced, which comprised 2,931,270 bp with G + C content of 66.5%. A supermatrix tree based on the gene set bac120 indicated that LY-0111 was close related to Nesterenkonia xinjiangensis YIM 70097 (16S rRNA gene sequence similarity 95.3%). Chemotaxonomic analysis indicated that the main respiratory quinones were MK-7, MK-8, and MK-9, the predominant cellular fatty acids were anteiso-C and iso-C, and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. According to the phenotypic, chemotaxonomic and phylogenetic features, strain LY-0111 is considered to represent a novel species, for which the name Nesterenkonia aerolata sp. nov. is proposed. The type strain is LY-0111 (= JCM 36375 = GDMCC 1.3945). In addition, Nesterenkonia jeotgali was proposed as a later synonym of Nesterenkonia sandarakina, according to the ANI (96.8%) and dDDH (72.9%) analysis between them.
在对中国山东省一家制药厂生产车间空气中微生物多样性的研究中，我们从沉降盘中分离到一株革兰氏阳性、需氧、球形细菌，命名为 LY-0111。菌株 LY-0111 在 10-42°C（最佳温度 35°C）、pH 值 5.0-10.0（最佳 pH 值 7.0）和 NaCl 浓度 1-12%（最佳 0.5-3%，w/v）的条件下生长。基于 16S rRNA 基因序列分析，该菌株与 Nesterenkonia halophila YIM 70179 的序列相似度最高（96.2%），并在系统发育树中处于 Nesterenkonia 种的辐射范围内。该分离株的基因组进行了测序，基因组大小为 2,931,270 bp，GC 含量为 66.5%。基于 bac120 基因集构建的超级矩阵树表明，LY-0111 与 Nesterenkonia xinjiangensis YIM 70097 关系较近（16S rRNA 基因序列相似度 95.3%）。化学分类分析表明，主要的呼吸醌为 MK-7、MK-8 和 MK-9，主要的细胞脂肪酸为 anteiso-C 和 iso-C，主要的极性脂由双磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇组成。根据表型、化学分类和系统发育特征，菌株 LY-0111 被认为代表一个新的种，命名为 Aerolata 属 Nesterenkonia aerolata sp. nov.。模式菌株为 LY-0111（=JCM 36375=GDMCC 1.3945）。此外，根据它们之间的 ANI（96.8%）和 dDDH（72.9%）分析，Nesterenkonia jeotgali 被提议作为 Nesterenkonia sandarakina 的后同物。
