Luo Hui-Ying, Miao Li-Hong, Fang Chengxiang, Yang Pei-Long, Wang Ya-Ru, Shi Peng-Jun, Yao Bin, Fan Yun-Liu
Microbial Engineering Department, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2008 Aug;58(Pt 8):1927-30. doi: 10.1099/ijs.0.65618-0.
A Gram-positive, non-motile, rod-shaped, non-spore-forming bacterium, designated CAAS 251T, was isolated from paper-mill effluent in Wuhan, China. The organism grew optimally at 40-42 degrees C and at pH 9.0-10.0. The major menaquinones were MK-7, MK-8 and MK-9. The predominant cellular fatty acids were anteiso-C15:0 (34.78 %), anteiso-C17:0 (25.24 %) and C16:0 (13.37 %). The G+C content of the genomic DNA was 65.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAAS 251T belongs to the genus Nesterenkonia, having sequence identities ranging from 96.0 to 97.0 % with respect to eight recognized species of the genus Nesterenkonia. Data from DNA-DNA hybridization and physiological and biochemical tests indicated that strain CAAS 251T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia flava sp. nov. is proposed. The type strain is CAAS 251T (=CCTCC AB 207010T=JCM 14814T).
从中国武汉的造纸厂废水中分离出一株革兰氏阳性、无运动性、杆状、不形成芽孢的细菌，命名为CAAS 251T。该菌株在40 - 42℃和pH 9.0 - 10.0条件下生长最佳。主要的甲基萘醌为MK - 7、MK - 8和MK - 9。主要的细胞脂肪酸为anteiso - C15:0（34.78%）、anteiso - C17:0（25.24%）和C16:0（13.37%）。基因组DNA的G + C含量为65.5 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株CAAS 251T属于涅斯捷连科氏菌属，与涅斯捷连科氏菌属已确认的8个种的序列相似性为96.0%至97.0%。DNA - DNA杂交以及生理生化试验的数据表明，菌株CAAS 251T代表涅斯捷连科氏菌属的一个新种，为此提出新种名为黄色涅斯捷连科氏菌（Nesterenkonia flava sp. nov.）。模式菌株为CAAS 251T（=CCTCC AB 207010T =JCM 14814T）。
