Suzuki Akemi, Bisordi Ivani, Levis Silvana, Garcia Jorge, Pereira Luiz E, Souza Renato P, Sugahara Teresa K N, Pini Noemi, Enria Delia, Souza Luiza T M
Instituto Adolfo Lutz-São Paulo, São Paulo, Brazil.
Emerg Infect Dis. 2004 Dec;10(12):2127-34. doi: 10.3201/eid1012.040295.
We describe the genetic analysis of samples from hantavirus pulmonary syndrome (HPS) patients from southern and southeastern states of Brazil and rodents captured at the presumed site of infection of these patients. A total of 65 samples that were antibody-positive for Sin Nombre or Laguna Negra virus by enzyme-linked immunosorbent assay were processed by nested reverse transcription-polymerase chain reaction (RT-PCR) by using several primer combinations in the M and S genome segments. PCR products were amplified and sequenced from samples from 11 HPS patient and 7 rodent samples. Phylogenetic analysis of nucleotide sequence differences showed the cocirculation of Araraquara and Juquitiba-like viruses, previously characterized from humans. Our genetic data indicate that Araraquara virus is associated with Bolomys lasiurus (hairy-tailed Bolo mouse) and the Juquitiba-like virus is associated with Oligoryzomys nigripes (black-footed pigmy rice rat).
我们描述了对来自巴西南部和东南部各州汉坦病毒肺综合征（HPS）患者的样本以及在这些患者推测感染地点捕获的啮齿动物进行的基因分析。通过酶联免疫吸附测定法对辛诺柏病毒或拉古纳内格拉病毒呈抗体阳性的总共65个样本，利用M和S基因组片段中的几种引物组合，通过巢式逆转录聚合酶链反应（RT-PCR）进行处理。从11份HPS患者样本和7份啮齿动物样本中扩增并测序了PCR产物。对核苷酸序列差异的系统发育分析显示，此前已从人类中鉴定出的阿拉拉夸拉病毒和类朱基蒂巴病毒共同传播。我们的基因数据表明，阿拉拉夸拉病毒与多毛尾波罗鼠（Bolomys lasiurus）有关，类朱基蒂巴病毒与黑足侏鼠（Oligoryzomys nigripes）有关。
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