Dinh Q Thai, Groneberg David A, Peiser Christian, Joachim Ricarda A, Frossard Nelly, Arck Petra C, Klapp Burghard F, Fischer Axel
Department of Internal Medicine, Charité School of Medicine, Humboldt University, Berlin, Germany.
Regul Pept. 2005 Mar 30;126(3):189-94. doi: 10.1016/j.regpep.2004.09.006.
Airway sensory nerves have the capacity to release neuromediators such as substance P and nitric oxide to control airway functions. The aim of the present study was to investigate substance P and neuronal nitric oxide synthase (NOS-1) expression in airway-specific sensory neurons.
Airway-projecting neurons in the jugular-nodose ganglia were investigated for NOS-1 and substance P expression by neuronal tracing and double-labelling immunoreactivity.
Of the Fast blue labelled neurons, 14.6+/-1.8% (mean+/-S.E.M.) were immunoreactive only for NOS-1, 3.0+/-0.3% for NOS-1 and substance P, 2.7+/-0.3% only for substance P, and 79.7+/-1.7% of the labelled neurons were nonimmunoreactive for substance P or NOS-1 but were partly positive for I-B4-lectin-binding. Fast blue labelled NOS and/or substance P-positive neurons were small to medium sized (<20 microm).
Based on the expression of substance P and nitric oxide synthase in airway neurons, the present study suggests that there may be substance P and NO biosynthesis and release following a peripheral activation of the afferents, there could be a triggering of substance P and NO-mediated phenomena, including those related to airway inflammation, such as plasma extravasation and vasodilatation.
气道感觉神经能够释放神经介质，如P物质和一氧化氮，以控制气道功能。本研究的目的是调查气道特异性感觉神经元中P物质和神经元型一氧化氮合酶（NOS-1）的表达情况。
通过神经元追踪和双标免疫反应性，研究颈静脉神经节中投射至气道的神经元的NOS-1和P物质表达。
在快蓝标记的神经元中，14.6±1.8%（平均值±标准误）仅对NOS-1呈免疫反应性，3.0±0.3%对NOS-1和P物质呈免疫反应性，2.7±0.3%仅对P物质呈免疫反应性，79.7±1.7%的标记神经元对P物质或NOS-1无免疫反应性，但对I-B4-凝集素结合呈部分阳性。快蓝标记的NOS和/或P物质阳性神经元为中小型（<20微米）。
基于气道神经元中P物质和一氧化氮合酶的表达，本研究表明，在外周传入神经激活后，可能存在P物质和一氧化氮的生物合成与释放，可能引发P物质和一氧化氮介导的现象，包括与气道炎症相关的现象，如血浆渗出和血管舒张。
