Wielgosz Matthew M, Rauch Daniel A, Jones Kathryn S, Ruscetti Frank W, Ratner Lee
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
AIDS Res Hum Retroviruses. 2005 Jan;21(1):43-50. doi: 10.1089/aid.2005.21.43.
Cholesterol-rich plasma membrane microdomains are important for entry of many viruses, including retroviruses. Depletion of cholesterol with 2-hydroxypropyl-beta-cyclodextrin inhibits entry of human T cell leukemia virus type I (HTLV-1) and HTLV-I envelope pseudotyped lentivirus particles. Using a soluble fusion protein of the HTLV-I surface envelope protein with the immunoglobulin Fc domain, the HTLV-I receptor was found to colocalize with a raft-associated marker and to cluster in specific plasma membrane microdomains. Depletion of cholesterol did not alter receptor binding activity, suggesting a requirement for cholesterol in a postbinding virus entry step.
富含胆固醇的质膜微结构域对包括逆转录病毒在内的许多病毒的进入都很重要。用2-羟丙基-β-环糊精消耗胆固醇可抑制I型人类T细胞白血病病毒（HTLV-1）和HTLV-I包膜假型慢病毒颗粒的进入。使用HTLV-I表面包膜蛋白与免疫球蛋白Fc结构域的可溶性融合蛋白，发现HTLV-I受体与筏相关标记共定位，并聚集在特定的质膜微结构域中。胆固醇的消耗并没有改变受体结合活性，这表明在病毒结合后的进入步骤中需要胆固醇。
