细菌病毒的冷冻电镜断层扫描。
Cryo-electron tomography of bacterial viruses.
机构信息
Division of Pediatric Infectious Diseases, Emory University School of Medicine, Children's Healthcare of Atlanta, Atlanta, GA 30322, USA.
出版信息
Virology. 2013 Jan 5;435(1):179-86. doi: 10.1016/j.virol.2012.08.022.
Abstract
Bacteriophage particles contain both simple and complex macromolecular assemblages and machines that enable them to regulate the infection process under diverse environmental conditions with a broad range of bacterial hosts. Recent developments in cryo-electron tomography (cryo-ET) make it possible to observe the interactions of bacteriophages with their host cells under native-state conditions at unprecedented resolution and in three-dimensions. This review describes the application of cryo-ET to studies of bacteriophage attachment, genome ejection, assembly and egress. Current topics of investigation and future directions in the field are also discussed.
摘要
噬菌体颗粒包含简单和复杂的大分子组装体和机器，使它们能够在不同的环境条件下，以广泛的细菌宿主为对象，调节感染过程。低温电子断层扫描（cryo-ET）的最新发展使得在前所未有的分辨率和三维空间中观察噬菌体与其宿主细胞在自然状态下的相互作用成为可能。本综述描述了 cryo-ET 在噬菌体附着、基因组排出、组装和出芽研究中的应用。还讨论了该领域当前的研究课题和未来的发展方向。
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