Vilcek S, Ridpath J F, Van Campen H, Cavender J L, Warg J
University of Veterinary Medicine, Department of Parasitology and Infectious Diseases, Komenskeho 73, 04181 Kosice, Slovakia.
Virus Res. 2005 Mar;108(1-2):187-93. doi: 10.1016/j.virusres.2004.09.010.
A unique pestivirus, isolated from a pronghorn antelope (Antilocopra americana), was characterized. Serum neutralization studies suggested that this virus was antigenically related to pestiviruses. Genomic characteristics, unique to pestiviruses, indicated that this virus belongs to the Pestivirus genus. These characteristics included the organization of the 5' untranslated region (5'-UTR), the presence and length of a viral Npro coding region, conservation of cysteine residues in Npro, conservation of predicted amino acid sequences flanking the cleavage sites between viral polypeptides Npro and C and between C and Erns and conservation of predicted hydrophobicity plots of Npro protein. While this data indicated the virus belongs to the Pestivirus genus, phylogenetic analysis in 5'-UTR, Npro and E2 regions suggested that it is the most divergent of the pestiviruses identified to date. This conclusion was also supported by the amino acid identity in coding regions. The corresponding values were much lower for the comparison of pronghorn pestivirus to other pestivirus genotypes than only between previous recognized genotypes. These results suggest the virus isolated from pronghorn antelope represents a new pestivirus genotype. It also represents the only pestivirus genotype first isolated from New World wildlife.
一种从叉角羚（美洲叉角羚）中分离出的独特瘟病毒得到了鉴定。血清中和试验表明，该病毒在抗原性上与瘟病毒相关。瘟病毒特有的基因组特征表明，该病毒属于瘟病毒属。这些特征包括5'非翻译区（5'-UTR）的结构、病毒Npro编码区的存在和长度、Npro中半胱氨酸残基的保守性、病毒多肽Npro与C之间以及C与Erns之间切割位点两侧预测氨基酸序列的保守性，以及Npro蛋白预测疏水性图谱的保守性。虽然这些数据表明该病毒属于瘟病毒属，但对5'-UTR、Npro和E2区域的系统发育分析表明，它是迄今为止已鉴定的瘟病毒中差异最大的。编码区的氨基酸同一性也支持了这一结论。与其他瘟病毒基因型相比，叉角羚瘟病毒的相应值要低得多，而此前仅在已确认的基因型之间进行比较时，相应值则没有这么低。这些结果表明，从叉角羚中分离出的病毒代表了一种新的瘟病毒基因型。它也是首个从新大陆野生动物中分离出的瘟病毒基因型。
