感染过程中血浆中严重急性呼吸综合征冠状病毒RNA的检测
Detection of severe acute respiratory syndrome coronavirus RNA in plasma during the course of infection.
作者信息
Wang Wei-Kung, Fang Chi-Tai, Chen Hui-Ling, Yang Chao-Fu, Chen Yee-Chun, Chen Mei-Ling, Chen Shey-Ying, Yang Jyh-Yuan, Lin Jih-Hui, Yang Pan-Chyr, Chang Shan-Chwen
机构信息
Institute of Microbiology, College of Medicine, National Taiwan University, Tapei, Taiwan.
出版信息
J Clin Microbiol. 2005 Feb;43(2):962-5. doi: 10.1128/JCM.43.2.962-965.2005.
We examined severe acute respiratory syndrome-associated coronavirus (SARS-CoV) RNA in plasma of 32 patients (probable SARS cases) by a quantitative real-time reverse transcription-PCR assay and reported that the highest detection rate, 75%, was found between day 5 and day 7 of illness, followed by rates of 64, 50, and 38% found between day 8 and day 11, day 2 and day 4, and day 12 and day 16, respectively. Analysis of sequential SARS-CoV load in plasma from six cases revealed different patterns of viremia, with the peak between day 4 and day 8. Our findings of the high detection rate of SARS-CoV RNA in plasma before day 11, together with the relative convenience of collecting and handling plasma, suggest that plasma can be used for early diagnosis of SARS.
我们通过定量实时逆转录聚合酶链反应检测法，对32例患者（疑似严重急性呼吸综合征病例）血浆中的严重急性呼吸综合征相关冠状病毒（SARS-CoV）RNA进行了检测，并报告称，在发病第5天至第7天期间检测率最高，为75%，其次在发病第8天至第11天、第2天至第4天以及第12天至第16天期间的检测率分别为64%、50%和38%。对6例患者血浆中SARS-CoV载量的连续分析显示出不同的病毒血症模式，峰值出现在第4天至第8天之间。我们发现在发病第11天之前血浆中SARS-CoV RNA的检测率较高，再加上采集和处理血浆相对方便，这表明血浆可用于严重急性呼吸综合征的早期诊断。
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