在感染患者血液中检测严重急性呼吸综合征冠状病毒
Detection of severe acute respiratory syndrome coronavirus in blood of infected patients.
作者信息
Ng Lisa F P, Wong Michelle, Koh Susie, Ooi Eng-Eong, Tang King-Fai, Leong Hoe-Nam, Ling Ai-Ee, Agathe Lora V, Tan Jenny, Liu Edison T, Ren Ee-Chee, Ng Lee-Ching, Hibberd Martin L
机构信息
Genome Institute of Singapore, Singapore, Republic of Singapore.
出版信息
J Clin Microbiol. 2004 Jan;42(1):347-50. doi: 10.1128/JCM.42.1.347-350.2004.
Abstract
Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.
摘要
严重急性呼吸综合征（SARS）已在全球范围内引发大规模疫情，因此迫切需要获得对该疾病敏感且准确的诊断方法。基于聚合酶链反应（PCR）的检测方法已被开发用于多种样本，包括血液。对18名受试者进行了调查，结果表明血液样本中含有足够的病毒，可通过定量实时PCR进行检测。
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