Kim Hae-Won, Lee Eun-Jung, Kim Hyoun-Ee, Salih Vehid, Knowles Jonathan C
School of Materials Science and Engineering, Seoul National University, Seoul 151-742, Korea.
Biomaterials. 2005 Jul;26(21):4395-404. doi: 10.1016/j.biomaterials.2004.11.008.
Fluorine was administered to a system of hydroxyapatite (HA)/polycaprolactone (PCL) ceramic-polymer bioactive composites for applications as hard tissue regeneratives. The HA was fluoridated at different levels (5%, 25%, 50% and 75%) in order to produce the fluor-hydroxyapatite (FHA)/PCL composites. The osteoblastic cellular responses to the composites were examined in terms of the cell attachment, proliferation and differentiation as well as the expression of bone-associated genes. The amount of fluorine released from the composites was controlled by changing the degree of fluoridation, and the cellular responses were strongly influenced by the level of fluoridation. The MG63 cells on the FHA-PCL attached and proliferated at a similar level to those on HA-PCL. However, the fluoridation of HA increased significantly the alkaline phosphatase (ALP) activity and osteocalcin (OC) production by the cells on the composites, which was measured by an enzymatic assay. Moreover, the gene expression level of ALP and OC in the cells was up regulated on the FHA-PCL, which was confirmed semi-quantitatively by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. These findings on the fluorine-administered biological composites (FHA-PCL) suggested that fluorine plays a significant role in stimulating the bone derived cellular activity, and the FHA-PCL composites have high potential for use as hard tissue regeneratives.
将氟应用于羟基磷灰石（HA）/聚己内酯（PCL）陶瓷-聚合物生物活性复合材料体系，以用于硬组织再生。对HA进行不同水平（5%、25%、50%和75%）的氟化处理，以制备氟羟基磷灰石（FHA）/PCL复合材料。从细胞附着、增殖和分化以及骨相关基因的表达方面研究了成骨细胞对复合材料的反应。通过改变氟化程度来控制复合材料释放的氟量，并且细胞反应受到氟化水平的强烈影响。FHA-PCL上的MG63细胞的附着和增殖水平与HA-PCL上的细胞相似。然而，HA的氟化显著增加了复合材料上细胞的碱性磷酸酶（ALP）活性和骨钙素（OC）生成，这通过酶促测定法进行测量。此外，通过逆转录聚合酶链反应（RT-PCR）分析半定量证实，FHA-PCL上细胞中ALP和OC的基因表达水平上调。这些关于含氟生物复合材料（FHA-PCL）的研究结果表明，氟在刺激骨源性细胞活性方面发挥着重要作用，并且FHA-PCL复合材料具有作为硬组织再生材料的巨大潜力。
