Wang Yongsheng, Zhang Sam, Zeng Xianting, Ma Lwin Lwin, Khor Khiam Aik, Qian Min
School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.
J Biomed Mater Res A. 2008 Mar 1;84(3):769-76. doi: 10.1002/jbm.a.31289.
Initial cell attachment and spreading of anchorage-dependent cells onto the material surface are crucial concerns for the development of more effective implants. In this study, MG63 cells were employed to investigate the initial cell response to sol-gel derived fluoridated hydroxyapatite (FHA) coatings. Along with that, surface roughness, wettability, and protein adsorption were also characterized for those FHA coatings, respectively. It was observed that both the surface roughness and contact angle have a slight increase in response to the incorporation of more fluorine ions. All FHA coatings showed similar amount of adsorbed proteins (approximately 1.6 microg/cm(2)) upon testing in culture medium. Cell counting showed that no significant difference was observed for the amount of initially attached cells between HA and fluoridated HA coatings during the first 4 h culture. On the other hand, the well-spread cell on all prepared coating surface indicates that the incorporated fluorine ions have no adverse effect on cell spreading process. Therefore, it was suggested from this study that the prepared fluoridated hydroxyapatite coatings have comparable bioactivity to that of pure hydroxyapatite coating, and these results are meaningful for further investigation for application of FHA coatings.
对于开发更有效的植入物而言，贴壁依赖性细胞在材料表面的初始细胞附着和铺展是至关重要的问题。在本研究中，采用MG63细胞来研究其对溶胶 - 凝胶法制备的含氟羟基磷灰石（FHA）涂层的初始细胞反应。与此同时，还分别对这些FHA涂层的表面粗糙度、润湿性和蛋白质吸附进行了表征。结果观察到，随着氟离子掺入量的增加，表面粗糙度和接触角均略有增加。在培养基中测试时，所有FHA涂层显示出相似量的吸附蛋白（约1.6μg/cm²）。细胞计数表明，在最初4小时的培养过程中，HA涂层和含氟HA涂层上初始附着细胞的数量未观察到显著差异。另一方面，在所有制备的涂层表面上细胞铺展良好，表明掺入的氟离子对细胞铺展过程没有不利影响。因此，本研究表明所制备的含氟羟基磷灰石涂层具有与纯羟基磷灰石涂层相当的生物活性，这些结果对于进一步研究FHA涂层的应用具有重要意义。
