Shires G Tom, Browder Leslie K, Steljes Trina P V, Williams Shelley J, Browder Timothy D, Barber Annabel E
Trauma Institute of UNSOM, University of Nevada School of Medicine, 2040 W. Charleston Blvd., Ste. 501, Las Vegas, NV 89102, USA.
Am J Surg. 2005 Jan;189(1):85-91. doi: 10.1016/j.amjsurg.2004.06.040.
Recent data suggest that the type of resuscitation fluid used to treat hemorrhagic shock contributes to cellular dysfunction
Rats were hemorrhaged, exposed to a hypovolemic shock period for 75 minutes, and then resuscitated for 1 hour. Treatment animals were assigned randomly to lactate Ringer's solution, normal saline solution, bicarbonate Ringer's solution, hypertonic saline solution, rat plasma solution, ketone Ringer's solution, or nonresuscitation. After resuscitation, lung and liver samples were collected and evaluated for apoptosis by using ligation-mediated polymerase chain reaction.
Nonresuscitated shock rats had significantly higher levels of apoptosis in lung and liver. Rats treated with normal saline solution, bicarbonate Ringer's solution, ketone Ringer's solution, and hypertonic saline solution had significantly lower levels of apoptosis in lung compared with nonresuscitated animals. Rats treated with bicarbonate Ringer's solution and ketone Ringer's solution had significantly lower levels of apoptosis in liver tissue when compared with nonresuscitated animals.
Cellular damage results from hemorrhagic shock. The use of resuscitation fluids decreases apoptosis during shock.
近期数据表明，用于治疗失血性休克的复苏液类型会导致细胞功能障碍。
将大鼠放血，使其经历75分钟的低血容量休克期，然后复苏1小时。将治疗动物随机分为接受乳酸林格氏液、生理盐水、碳酸氢盐林格氏液、高渗盐溶液、大鼠血浆溶液、酮林格氏液治疗或不进行复苏处理。复苏后，收集肺和肝组织样本，采用连接介导的聚合酶链反应评估细胞凋亡情况。
未进行复苏的休克大鼠肺和肝组织中的细胞凋亡水平显著更高。与未复苏动物相比，接受生理盐水、碳酸氢盐林格氏液、酮林格氏液和高渗盐溶液治疗的大鼠肺组织中的细胞凋亡水平显著更低。与未复苏动物相比，接受碳酸氢盐林格氏液和酮林格氏液治疗的大鼠肝组织中的细胞凋亡水平显著更低。
失血性休克会导致细胞损伤。使用复苏液可减少休克期间的细胞凋亡。
