Chang J E, Wong J T
Can J Biochem. 1977 Apr;55(4):398-403. doi: 10.1139/o77-055.
Experimental conditions were defined that determined the synthesis of dextran-hemoglobin complexes through the alklation of hemoglobin by N-bromoacetylaminoethylaminodextran. Using appropriate concentrations of the two reactants, over 90% yield of dextran-hemoglobin was obtained for dextrans of average molecular weight of 200 000 110000, 70000, 400000, and 20000. Extensive viscosity increase due to crosslinking could be avoided, and a large molar excess of dextran over hemoglobin made unnecessary, under the optimal conditions.
确定了通过N-溴乙酰氨基乙基氨基葡聚糖对血红蛋白进行烷基化反应来合成葡聚糖-血红蛋白复合物的实验条件。使用两种反应物的适当浓度，对于平均分子量为200000、110000、70000、40000和20000的葡聚糖，葡聚糖-血红蛋白的产率超过90%。在最佳条件下，可以避免由于交联导致的粘度大幅增加，并且不需要葡聚糖相对于血红蛋白有很大的摩尔过量。
