Cunnington P G, Jenkins S N, Tam S C, Wong J T
Biochem J. 1981 Jan 1;193(1):261-6. doi: 10.1042/bj1930261.
Complexes of dextran 20 000 with haemoglobins of sheep, rabbit, dog, bovine and human origin were prepared through alkylation of haemoglobin by N-bromoacetylaminoethylamino-dextran. The yields were uniformly high. Complex-formation in each case was accompanied by the disappearance of reactive thiol groups on the haemoglobin, and by an increase in the affinity of the haemoglobin for oxygen. The immunological properties of dog, rabbit and sheep dextran-haemoglobin were investigated in both homologous and heterologous species. The complexes were found to be non-immunogenic in the homologous species. In heterologous species the anti-haemoglobin response induced by each complex was generally of a similar level to that induced by the haemoglobin alone.
通过用N-溴乙酰氨基乙氨基葡聚糖对血红蛋白进行烷基化反应，制备了与绵羊、兔子、狗、牛和人源血红蛋白结合的20000葡聚糖复合物。产率一直很高。在每种情况下，复合物的形成都伴随着血红蛋白上反应性巯基的消失，以及血红蛋白对氧气亲和力的增加。对狗、兔子和绵羊的葡聚糖-血红蛋白的免疫特性在同源和异源物种中进行了研究。发现这些复合物在同源物种中无免疫原性。在异源物种中，每种复合物诱导的抗血红蛋白反应通常与单独的血红蛋白诱导的反应水平相似。
