Harjai Kusum, Mittal Rahul, Chhibber Sanjay, Sharma Saroj
Department of Microbiology, Biomedical Sciences Block, Panjab University, Chandigarh 160014, India.
Microbes Infect. 2005 Jan;7(1):132-7. doi: 10.1016/j.micinf.2004.09.005. Epub 2004 Nov 24.
Tamm-Horsfall glycoprotein (THP) is the most abundant protein which is synthesized by renal tubular cells and excreted in urine. Its role in urinary tract infection has yet not been identified. In the present study, the contribution of THP towards adherence of Pseudomonas aeruginosa to uroepithelial cells and murine peritoneal macrophages was studied. Decreased adherence of THP-coated P. aeruginosa to UECs and phagocytes was observed in vitro. In vivo, P. aeruginosa showed increased renal bacterial load and tissue pathology in a mouse model of acute ascending pyelonephritis, when THP-coated P. aeruginosa was used to cause infection. This study shows that THP may not necessarily act as a host defense component; rather, it may help in renal colonization of P. aeruginosa in vivo.
Tamm-Horsfall糖蛋白（THP）是由肾小管细胞合成并随尿液排出的最丰富的蛋白质。其在尿路感染中的作用尚未明确。在本研究中，研究了THP对铜绿假单胞菌黏附于尿道上皮细胞和小鼠腹膜巨噬细胞的影响。体外观察到包被THP的铜绿假单胞菌对尿道上皮细胞和吞噬细胞的黏附减少。在体内，当使用包被THP的铜绿假单胞菌引起感染时，在急性上行性肾盂肾炎小鼠模型中，铜绿假单胞菌的肾脏细菌载量增加且组织病理学改变加重。本研究表明，THP不一定作为宿主防御成分发挥作用；相反，它可能有助于铜绿假单胞菌在体内定殖于肾脏。
