Mittal Rahul, Sharma Saroj, Chhibber Sanjay, Harjai Kusum
Department of Microbiology, Panjab University, BAMS Block, Chandigarh 160014, India.
Comp Immunol Microbiol Infect Dis. 2006 Jan;29(1):12-26. doi: 10.1016/j.cimid.2005.11.002. Epub 2006 Jan 20.
Macrophages, which constitute the first line of defense, pour their secretions in the mileu following stimulation with pathogens. These secretory products, referred to as macrophage secretory products (MSPs), can influence ultimate outcome of an infection. In the present investigation, it was observed that different strains of Pseudomonas aeruginosa vary in their ability to stimulate macrophages leading to variability in generation of macrophage secretory products. Cytokine levels, reactive nitrogen intermediates and protein content of macrophage secretory products generated with biofilm cells of P. aeruginosa was found to be more as compared to their planktonic counterparts. The effect of macrophage secretory products produced in response to interaction of macrophages with P. aeruginosa on elaboration of virulence factors produced by planktonic and biofilm cell forms of this pathogen was assessed. Significant enhancement in growth and elaboration of all the virulence determinants by both the cell forms was observed when P. aeruginosa was grown in presence of supernatants with macrophage secretory products. Implications of these findings in relation to urinary tract infections induced by P. aeruginosa have been discussed.
巨噬细胞构成了第一道防线，在受到病原体刺激后会向周围环境中分泌物质。这些分泌产物被称为巨噬细胞分泌产物（MSPs），它们能够影响感染的最终结果。在本研究中，观察到不同菌株的铜绿假单胞菌刺激巨噬细胞的能力存在差异，导致巨噬细胞分泌产物的产生也有所不同。与浮游菌相比，铜绿假单胞菌生物被膜细胞产生的巨噬细胞分泌产物中的细胞因子水平、活性氮中间体和蛋白质含量更高。评估了巨噬细胞与铜绿假单胞菌相互作用产生的巨噬细胞分泌产物对该病原体浮游菌和生物被膜细胞形式产生的毒力因子的影响。当铜绿假单胞菌在含有巨噬细胞分泌产物的上清液中生长时，观察到两种细胞形式的所有毒力决定因素的生长和产生均显著增强。讨论了这些发现与铜绿假单胞菌引起的尿路感染的关系。
