Freeman M, Weetman A P
Department of Medicine, University of Sheffield, Northern General Hospital, UK.
Clin Exp Immunol. 1992 May;88(2):275-9. doi: 10.1111/j.1365-2249.1992.tb03073.x.
Autoimmune Addison's disease is a rare condition of uncertain pathogenesis. To delineate potential autoantigens, an adrenal homogenate was fractioned by SDS-gel electrophoresis and used in immunoblotting and T cell proliferation assays. Antibodies specific for adrenal proteins with approximate molecular weights of 70, 55 and 45 were found in five out of 20 consecutive Addison's disease patients at routine follow-up. Five sera also reacted with a 52-kD protein shared with liver. T cells from six out of 10 Addison's disease patients proliferated in response to a range of adrenal-specific antigens including one, in particular, with a molecular weight of 18-24 kD. T and B cell reactivity to adrenal antigens did not appear to correlate and there was no relationship with time since diagnosis, associated autoimmunity or HLA-DR type. These results show that the autoimmune response to adrenal antigens in Addison's disease is heterogeneous and that such autoreactivity can only be inconsistently documented using these techniques and circulating antibodies or T cells.
自身免疫性艾迪生病是一种发病机制不明的罕见疾病。为了确定潜在的自身抗原，将肾上腺匀浆进行十二烷基硫酸钠凝胶电泳分离，并用于免疫印迹和T细胞增殖试验。在20例连续的艾迪生病患者的常规随访中，有5例发现了对分子量约为70、55和45的肾上腺蛋白具有特异性的抗体。5份血清还与一种肝脏共有的52-kD蛋白发生反应。10例艾迪生病患者中有6例的T细胞对一系列肾上腺特异性抗原产生增殖反应，其中特别是一种分子量为18 - 24 kD的抗原。T细胞和B细胞对肾上腺抗原的反应性似乎不相关，且与诊断后的时间、相关自身免疫或HLA - DR类型无关。这些结果表明，艾迪生病中对肾上腺抗原的自身免疫反应是异质性的，并且使用这些技术以及循环抗体或T细胞只能不一致地记录这种自身反应性。
