Fox C J, Taylor M I, Pereyra R, Villasana M I, Rico C
The Centre for Environment, Fisheries and Aquaculture Science, Pakefield Road, Lowestoft, Suffolk, United Kingdom, NR33 OHT.
Mol Ecol. 2005 Mar;14(3):879-84. doi: 10.1111/j.1365-294X.2005.02439.x.
Recent substantial declines in northeastern Atlantic cod stocks necessitate improved biological knowledge and the development of techniques to complement standard stock assessment methods (which largely depend on accurate commercial catch data). In 2003, an ichthyoplankton survey was undertaken in the Irish Sea and subsamples of 'cod-like' eggs were analysed using a TaqMan multiplex, PCR (polymerase chain reaction) assay (with specific probes for cod, haddock and whiting). The TaqMan method was readily applied to the large number of samples (n = 2770) generated during the survey and when combined with a manual DNA extraction protocol had a low failure rate of 6%. Of the early stage 'cod-like' eggs (1.2-1.75 mm diameter) positively identified: 34% were cod, 8% haddock and 58% whiting. As previous stock estimates based on egg surveys for Irish Sea cod assumed that the majority of 'cod-like' eggs were from cod, the TaqMan results confirm that there was probably substantial contamination by eggs of whiting and haddock that would have inflated estimates of the stock biomass.
近期东北大西洋鳕鱼种群数量大幅下降，这就需要增进生物学知识，并开发相关技术以补充标准种群评估方法（标准种群评估方法很大程度上依赖准确的商业捕捞数据）。2003年，在爱尔兰海进行了一次鱼卵浮游生物调查，并使用TaqMan多重聚合酶链反应（PCR）分析方法（使用针对鳕鱼、黑线鳕和牙鳕的特异性探针）对“类鳕鱼”鱼卵的子样本进行了分析。TaqMan方法很容易应用于调查期间采集的大量样本（n = 2770），并且与手动DNA提取方案相结合时，失败率很低，仅为6%。在已确定的早期“类鳕鱼”鱼卵（直径1.2 - 1.75毫米）中：34%为鳕鱼，8%为黑线鳕，58%为牙鳕。由于之前基于爱尔兰海鳕鱼鱼卵调查的种群估计假设大多数“类鳕鱼”鱼卵来自鳕鱼，TaqMan方法的结果证实，可能存在大量牙鳕和黑线鳕的鱼卵污染，这会使种群生物量估计值虚增。
