Karrow N A, McCay J A, Brown R D, Musgrove D L, Guo T L, Germolec D R, White K L
Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298-6013, USA.
Toxicology. 2005 Apr 1;209(1):15-28. doi: 10.1016/j.tox.2004.12.002.
Atrazine (ATZ) is used throughout North America to control annual broadleaf weeds and grasses in various crops including; corn, sorghum, and sugar cane. Unfortunately, contamination of surface and ground water has occurred as a result of ATZ's chemical and physical properties, and its widespread use throughout the U.S. Midwest. A study of ATZ's immunomodulatory properties was conducted using female B6C3F1 mice and a panel of immune assays and host resistance models designed to evaluate cell-mediated and antibody-mediated immunity. Mice were administered ATZ by gavage (0, 24, 250, and 500 mg/kg/day) for 14 days then evaluated for immune responsiveness. ATZ treatment significantly increased the number of splenic CD8+ T cells, cytotoxic T cell and mixed leukocyte responses, and dose-dependently reduced host resistance to B16F10 melanoma. Thymus and spleen weights, total spleen cell numbers and fixed macrophage function was also reduced in mice that were exposed to ATZ. These results demonstrate that oral ATZ exposure is sufficient to alter cell-mediated immune function and disease resistance in female B6C3F1 mice.
阿特拉津（ATZ）在北美各地用于控制包括玉米、高粱和甘蔗在内的各种作物中的一年生阔叶杂草和禾本科杂草。不幸的是，由于阿特拉津的化学和物理性质及其在美国中西部的广泛使用，地表水和地下水已受到污染。使用雌性B6C3F1小鼠以及一组旨在评估细胞介导免疫和抗体介导免疫的免疫测定和宿主抗性模型，对阿特拉津的免疫调节特性进行了研究。通过灌胃给予小鼠阿特拉津（0、24、250和500毫克/千克/天），持续14天，然后评估其免疫反应性。阿特拉津处理显著增加了脾脏CD8 + T细胞的数量、细胞毒性T细胞和混合淋巴细胞反应，并剂量依赖性地降低了宿主对B16F10黑色素瘤的抗性。暴露于阿特拉津的小鼠的胸腺和脾脏重量、脾脏细胞总数和固定巨噬细胞功能也有所降低。这些结果表明，口服阿特拉津足以改变雌性B6C3F1小鼠的细胞介导免疫功能和抗病能力。
