Maldonado Luis A, Stach James E M, Pathom-aree Wasu, Ward Alan C, Bull Alan T, Goodfellow Michael
School of Biology, University of Newcastle, NE1 7RU, Newcastle upon Tyne, UK.
Antonie Van Leeuwenhoek. 2005 Jan;87(1):11-8. doi: 10.1007/s10482-004-6525-0.
Reports describing actinobacteria isolated from marine environments have been dominated by Micromonospora, Rhodococcus and Streptomyces species. Recent culture-independent studies have shown that marine environments contain a high diversity of actinobacterial species that are rarely, if at all, recovered by cultivation-based methods. In this study, it is shown that cultivation-independent methods can be used to guide the application of selective isolation methods. The detection of marine-derived actinobacterial species that have previously only been reported from terrestrial habitats is highlighted. This study provides good evidence that the previously described low diversity of actinobacterial species isolated from marine environments does not reflect an actual low species diversity, and that the use of informed selective isolation procedures can aid in the isolation of members of novel taxa.
描述从海洋环境中分离出的放线菌的报告主要集中在小单孢菌属、红球菌属和链霉菌属。最近基于非培养的研究表明，海洋环境中含有高度多样的放线菌物种，而基于培养的方法很少（如果有的话）能分离到这些物种。在本研究中，结果表明基于非培养的方法可用于指导选择性分离方法的应用。本文强调了对以前仅在陆地栖息地报道过的海洋来源放线菌物种的检测。这项研究提供了有力证据，表明先前描述的从海洋环境中分离出的放线菌物种多样性较低，并不能反映实际的低物种多样性，而且使用明智的选择性分离程序有助于分离新分类群的成员。
