Derry J M, Barnard P J
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, United Kingdom.
Genomics. 1992 Apr;12(4):632-8. doi: 10.1016/0888-7543(92)90286-2.
Genes encoding the neuron-specific phosphoprotein synapsin I (SYN1), the glycoprotein tissue inhibitor of metalloproteinases (TIMP), the proto-oncogene A-raf-1 (ARAF1), and properdin (PFC), a positive regulator of the alternative pathway of human complement, lie within a conserved synteny encompassing the proximal short arm of the human X chromosome (Xp21.1-p11) and the centromeric end of the mouse X chromosome (A1-A5). We have used a mouse interspecific cross to demonstrate genetic linkage of Syn-1, Timp, and Araf and also show physical linkage, with Timp lying only 10 kb from Araf, within an intron of the Syn-1 gene. Detailed restriction mapping shows that Timp is transcribed in the same direction as Araf but in the opposite direction to the Syn-1 gene. Analysis of the corresponding region of the human X chromosome indicates a similar arrangement and in addition shows that the properdin gene lies within 5 kb of the 5' end of the synapsin I gene.
编码神经元特异性磷蛋白突触素I（SYN1）、金属蛋白酶组织抑制剂糖蛋白（TIMP）、原癌基因A-raf-1（ARAF1）以及备解素（PFC，人类补体替代途径的正向调节因子）的基因，位于一个保守的同线性区域内，该区域包括人类X染色体短臂近端（Xp21.1-p11）和小鼠X染色体着丝粒末端（A1-A5）。我们利用小鼠种间杂交来证明Syn-1、Timp和Araf的遗传连锁，并且还显示了物理连锁，其中Timp距离Araf仅10 kb，位于Syn-1基因的一个内含子内。详细的限制性图谱分析表明，Timp与Araf的转录方向相同，但与Syn-1基因的转录方向相反。对人类X染色体相应区域的分析表明存在类似的排列方式，此外还显示备解素基因位于突触素I基因5'端的5 kb范围内。
