Marczynski B, Preuss R, Mensing T, Angerer J, Seidel A, El Mourabit A, Wilhelm M, Brüning T
Research Institute of Occupational Medicine, Ruhr University of Bochum, Bürkle-de-la-Camp-Platz 1, 44789 Bochum, Germany.
Int Arch Occup Environ Health. 2005 Mar;78(2):97-108. doi: 10.1007/s00420-004-0567-5. Epub 2005 Feb 22.
Workers in various industries can be exposed to polycyclic aromatic hydrocarbons (PAHs). The relationship between biomarkers of genotoxic risk, PAH compounds in air (ambient monitoring) and PAH metabolites in urine (internal exposure) were studied in 17 workers exposed to PAHs in a fireproof-material producing plant before and 3 months after the PAH profile was altered in the binding pitch.
Two biomarkers of exposure, specific DNA adducts of (+/-)-r-7,t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (anti-BPDE) and non-specific DNA adduct of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) were determined in white blood cells (WBCs). In addition, DNA strand breaks were analysed in lymphocytes by single-cell gel electrophoresis in a genotoxic risk assessment. Sixteen PAH compounds in air were determined by personal air sampling, and hydroxylated metabolites of phenanthrene, pyrene and naphthalene were determined in urine.
After substitution of the binding pitch the concentrations of benzo[a]pyrene in air decreased (P<0.01). No changes could be observed for pyrene, while levels of phenanthrene (P=0.0013) and naphthalene (P=0.0346) in air increased. Consequently, median DNA adduct rates of anti-BPDE decreased after alteration of the production material (from 0.9 to <0.5 adducts/10(8) nucleotides). No changes in the excretion of 1-hydroxypyrene in urine could be determined, whereas increased levels of 1-, 2+9-, 3- and 4-hydroxyphenanthrene (P<0.0001) and 1-naphthol and 2-naphthol (P=0.0072) were found in urine. In addition, a statistically significant increase in DNA strand break frequencies (P<0.01) and elevated 8-oxodGuo adduct levels (P=0.7819, not statistically significant) were found in the WBCs of exposed workers 3 months after the PAH profile in the binding pitch had been altered.
The results presented here show that the increased concentration of naphthalene and/or phenanthrene in the air at the work place could induce the formation of DNA strand breaks and alkali-labile sites in WBCs of exposed workers.
不同行业的工人可能接触多环芳烃（PAHs）。对一家防火材料生产厂17名接触PAHs的工人进行研究，观察在结合沥青中PAH谱改变之前及之后3个月，遗传毒性风险生物标志物、空气中PAH化合物（环境监测）与尿液中PAH代谢物（体内暴露）之间的关系。
在白细胞（WBCs）中测定两种暴露生物标志物，即（±）-r-7，t-8-二羟基-t-9，10-环氧-7，8，9，10-四氢苯并[a]芘（反式BPDE）的特异性DNA加合物和8-氧代-7，8-二氢-2'-脱氧鸟苷（8-氧代dGuo）的非特异性DNA加合物。此外，在遗传毒性风险评估中，通过单细胞凝胶电泳分析淋巴细胞中的DNA链断裂情况。通过个人空气采样测定空气中16种PAH化合物，并测定尿液中菲、芘和萘的羟基化代谢物。
更换结合沥青后，空气中苯并[a]芘的浓度降低（P<0.01）。芘的浓度未观察到变化，而空气中菲（P=0.0013）和萘（P=0.0346）的浓度升高。因此，更换生产材料后，反式BPDE的DNA加合物中位率降低（从0.9降至<0.5个加合物/10^8个核苷酸）。尿液中1-羟基芘的排泄量未发现变化，而尿液中1-、2+9-、3-和4-羟基菲（P<0.0001）以及1-萘酚和2-萘酚（P=0.0072）的水平升高。此外，在结合沥青中PAH谱改变3个月后，暴露工人的白细胞中DNA链断裂频率有统计学显著增加（P<0.01），8-氧代dGuo加合物水平升高（P=0.7819，无统计学显著性）。
此处呈现的结果表明，工作场所空气中萘和/或菲浓度的增加可能会诱导暴露工人白细胞中DNA链断裂和碱不稳定位点的形成。
