Sato Hajime, Tomita Hiroshi, Nakazawa Toru, Wakana Shigeharu, Tamai Makoto
Department of Ophthalmology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
Invest Ophthalmol Vis Sci. 2005 Mar;46(3):791-6. doi: 10.1167/iovs.04-0867.
To characterize a novel gene, deleted in polyposis 1-like 1 (Dp1l1), which is expressed in the retina.
A systematic screening by subtraction hybridization of the cDNAs from mouse retina and mouse brain was performed to obtain novel genes expressed in the retina. In situ hybridization, immunohistochemistry, and intracellular localization analyses were performed to investigate the expression patterns of Dp1l1. The chromosomal location of Dp1l1 was determined by radiation hybrid mapping. Bioinformatics was used for homology analysis.
A novel gene, Dp1l1, was expressed abundantly in the retina. It encodes a 201-amino-acid protein, and the encoded protein is designated mouse TB2-like 1. It is highly homologous to the mouse TB2, which is encoded by deleted in polyposis 1 (Dp1). In situ hybridization and immunohistochemical analyses showed that Dp1l1 mRNA and the TB2-like 1 were localized richly in retinal ganglion cells (RGCs). TB2-like 1 was present in the cytoplasm in a punctate pattern. Dp1l1 was mapped to mouse chromosome 10 by radiation hybrid mapping.
TB2-like 1 is a membrane protein that belongs to the YOP1/TB2/DP1/HVA22 family, and it probably plays an important role in intracellular membrane trafficking in RGCs, based on the properties of other homologous proteins.
鉴定一种在视网膜中表达的新基因——息肉病1样1缺失基因（Dp1l1）。
通过对小鼠视网膜和小鼠脑cDNA进行消减杂交进行系统筛选，以获得在视网膜中表达的新基因。进行原位杂交、免疫组织化学和细胞内定位分析，以研究Dp1l1的表达模式。通过辐射杂种图谱确定Dp1l1的染色体定位。利用生物信息学进行同源性分析。
一个新基因Dp1l1在视网膜中大量表达。它编码一种含201个氨基酸的蛋白质，该编码蛋白被命名为小鼠TB2样1。它与由息肉病1（Dp1）缺失所编码的小鼠TB2高度同源。原位杂交和免疫组织化学分析表明，Dp1l1 mRNA和TB2样1在视网膜神经节细胞（RGCs）中大量定位。TB2样1以点状模式存在于细胞质中。通过辐射杂种图谱将Dp1l1定位到小鼠10号染色体。
基于其他同源蛋白的特性，TB2样1是一种属于YOP1/TB2/DP1/HVA22家族的膜蛋白，它可能在RGCs的细胞内膜运输中起重要作用。
