通过间接荧光抗体染色和放射免疫测定法对沙眼衣原体进行免疫分型的比较。
Comparison of immunotyping of Chlamydia trachomatis by indirect fluorescent-antibody staining and radioimmunoassay.
作者信息
van der Pol B J, Jones R B
机构信息
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202.
出版信息
J Clin Microbiol. 1992 Apr;30(4):1014-5. doi: 10.1128/jcm.30.4.1014-1015.1992.
Abstract
Chlamydia trachomatis isolates from 58 patients were immunotyped by radioimmunoassay (RIA) and fluorescent-antibody staining (FA) by using monoclonal antibodies. Unambiguous typing was observed with 55 isolates by FA and 53 isolates by RIA, although 3 isolates were typed by RIA but not FA. FA required less antigen and was more sensitive in detecting mixed infections.
摘要
采用单克隆抗体，通过放射免疫测定法（RIA）和荧光抗体染色法（FA）对58例患者的沙眼衣原体分离株进行免疫分型。通过FA对55株分离株进行了明确分型，通过RIA对53株分离株进行了明确分型，尽管有3株分离株通过RIA分型但未通过FA分型。FA所需抗原较少，在检测混合感染方面更敏感。
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