肺炎衣原体原体与荧光抗体的抗原反应性会被甲醇破坏。
Chlamydia pneumoniae elementary body antigenic reactivity with fluorescent antibody is destroyed by methanol.
作者信息
Wang S P, Grayston J T
机构信息
Department of Pathobiology, University of Washington, Seattle 98195.
出版信息
J Clin Microbiol. 1991 Jul;29(7):1539-41. doi: 10.1128/jcm.29.7.1539-1541.1991.
Abstract
Methanol, used for fixing chlamydia organisms to microscopic slides, was shown to eliminate the antigenic reactivity of Chlamydia pneumoniae elementary bodies with fluorescent antibodies. Acetone fixation had no adverse effect. Chlamydia trachomatis and Chlamydia psittaci were not affected by methanol fixation. The effect of methanol on C. pneumoniae is another characteristic that differentiates this species from the other two chlamydia species.
摘要
用于将衣原体固定到显微镜载玻片上的甲醇，已被证明会消除肺炎衣原体原体与荧光抗体的抗原反应性。丙酮固定没有不良影响。沙眼衣原体和鹦鹉热衣原体不受甲醇固定的影响。甲醇对肺炎衣原体的作用是将该物种与其他两种衣原体物种区分开来的另一个特征。
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