Schmidt Mark S, Prisinzano Thomas E, Tidgewell Kevin, Harding Wayne, Butelman Eduardo R, Kreek Mary J, Murry Daryl J
College of Pharmacy, Division of Clinical and Administrative Pharmacy, University of Iowa, Iowa City, IA 52242, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Apr 25;818(2):221-5. doi: 10.1016/j.jchromb.2004.12.041.
Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2 ng/mL for 0.5 mL plasma samples. The linear range was from 2 to 1000 ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d(3)-Salvinorin A was prepared and used as internal standard. The metabolite Salvinorin B can be semi quantitatively determined. The method has been used to establish that Salvinorin B is the principal metabolite of Salvinorin A ex vivo and to establish the analytical method to study in vivo samples.
通过负离子液相色谱-大气压化学电离质谱法（LC-MS/APCI）对人及恒河猴血浆、恒河猴脑脊液和人尿液中的萨尔维诺林A进行定量分析。萨尔维诺林A的分析方法已得到充分验证，按照美国食品药品监督管理局（FDA）指南，0.5 mL血浆样本的最低定量限（LLOQ）为2 ng/mL。线性范围为2至1000 ng/mL。该方法还可分析萨尔维诺林家族中的几种衍生物；制备了氘代-3-萨尔维诺林A（d(3)-Salvinorin A）并用作内标。代谢物萨尔维诺林B可进行半定量测定。该方法已用于确定萨尔维诺林B是萨尔维诺林A在体外的主要代谢物，并建立了用于研究体内样本的分析方法。
