Nigro Kelly G, O'Riordan MaryAnn, Molloy Eleanor J, Walsh Michele C, Sandhaus Linda M
Department of Pathology, Case Western Reserve University School of Medicine and University, Hospitals of Cleveland, Cleveland, OH 44106, USA.
Am J Clin Pathol. 2005 Apr;123(4):618-24. doi: 10.1309/73H7-K7UB-W816-PBJJ.
Neonatologists use immature granulocytes (IG) in manual differential counts as an indicator of sepsis. This study was designed to compare the predictive ability of automated vs manual IG counts for neonatal sepsis. Infants undergoing sepsis evaluation were identified prospectively for study if a CBC count was obtained in temporal proximity to the blood culture. Automated IG counts were obtained from the research software of the Sysmex XE-2100 (Sysmex, Kobe, Japan). Manual average IG counts were obtained from two 100-cell manual differential counts independently performed by a technologist and a hematopathology resident. A comparative analysis of manual and automated IG counts showed considerable overlap of ranges. The highest positive blood culture rate occurred in the nonneutropenic preterm subset of infant older than 7 days (21/55 [38%]). For these infants, elevated IG counts by manual and automated methods were associated significantly with positive blood culture results (odds ratio, manual, 3.74; odds ratio, automated, 3.63), albeit with low sensitivity.
新生儿科医生在手工分类计数中使用未成熟粒细胞（IG）作为败血症的指标。本研究旨在比较自动计数与手工计数IG对新生儿败血症的预测能力。如果在与血培养时间接近时获得全血细胞计数（CBC），则前瞻性地确定接受败血症评估的婴儿纳入研究。自动IG计数来自Sysmex XE - 2100（Sysmex，日本神户）的研究软件。手工平均IG计数由一名技术人员和一名血液病理学住院医师独立进行的两次100细胞手工分类计数获得。手工计数和自动计数IG的比较分析显示范围有相当大的重叠。最高的血培养阳性率出现在7天以上婴儿的非中性粒细胞减少早产儿亚组中（21/55 [38%]）。对于这些婴儿，手工和自动方法检测到的IG计数升高与血培养阳性结果显著相关（优势比，手工，3.74；优势比，自动，3.63），尽管敏感性较低。
