Engberg J, Bang D D, Aabenhus R, Aarestrup F M, Fussing V, Gerner-Smidt P
Unit of Gastrointestinal Infections, Statens Serum Institut, Copenhagen, Denmark.
Clin Microbiol Infect. 2005 Apr;11(4):288-95. doi: 10.1111/j.1469-0691.2005.01111.x.
The clinical relevance of Campylobacter concisus in gastrointestinal disease has not been determined definitively. This study investigated the phenotypic and genotypic characteristics of 39 C. concisus isolates from Danish patients with diarrhoea, three isolates from healthy individuals and the type strain. A cytolethal distending toxin (CDT)-like effect on Vero cells was observed in 35 (90%) isolates from patients with diarrhoea, in all three isolates from healthy individuals and in the type strain. Analysis of SDS-PAGE protein profiles and PCR amplification of 23S rDNA assigned the isolates into two distinct, but discordant groups. Automated ribotyping (RiboPrinting) identified 34 distinct patterns among the 43 isolates, but cluster analysis did not separate isolates from patients with diarrhoea from isolates from healthy patients. Random amplified polymorphic DNA (RAPD) analysis with three primers identified 37 unique profiles, but requires further evaluation. The isolates obtained from healthy carriers were distinguished by cluster analysis from the isolates obtained from patients with diarrhoea. All the isolates were susceptible to 11 antimicrobial agents tested. Overall, there was considerable variability between the C. concisus isolates, but there were no clear phenotypic or genotypic differences between isolates from patients with diarrhoea and isolates from healthy carriers. Further evidence is needed to support the possible role of C. concisus as a human enteric pathogen.
简明弯曲杆菌在胃肠道疾病中的临床相关性尚未明确确定。本研究调查了从丹麦腹泻患者中分离出的39株简明弯曲杆菌、3株健康个体分离株以及模式菌株的表型和基因型特征。在35株（90%）腹泻患者分离株、所有3株健康个体分离株以及模式菌株中均观察到对Vero细胞的细胞致死性扩张毒素（CDT）样效应。通过SDS-PAGE蛋白谱分析和23S rDNA的PCR扩增，将分离株分为两个不同但不一致的组。自动核糖体分型（RiboPrinting）在43株分离株中鉴定出34种不同模式，但聚类分析并未将腹泻患者的分离株与健康患者的分离株区分开来。用三种引物进行的随机扩增多态性DNA（RAPD）分析鉴定出37种独特谱型，但需要进一步评估。通过聚类分析，从健康携带者获得的分离株与从腹泻患者获得的分离株得以区分。所有分离株对所测试的11种抗菌药物均敏感。总体而言，简明弯曲杆菌分离株之间存在相当大的变异性，但腹泻患者的分离株与健康携带者的分离株之间没有明显的表型或基因型差异。需要进一步的证据来支持简明弯曲杆菌作为人类肠道病原体的可能作用。
