Kipar A, Baptiste K, Meli M L, Barth A, Knietsch M, Reinacher M, Lutz H
Institut für Veterinär-Pathologie, Justus-Liebig-Universität Giessen, Giessen, Germany.
Exp Gerontol. 2005 Mar;40(3):243-8. doi: 10.1016/j.exger.2004.12.007. Epub 2005 Jan 18.
Monocytes/macrophages are central mediators of inflammation and immunity and therefore of major interest in the study of immunosenescence. In healthy adult cats, monocytes have been shown to constitutively transcribe pro- and anti-inflammatory cytokines. However, in order to characterize the effect of age, feline monocyte functions were examined for changes in cytokine transcription levels in early stages of immunosenescence. For this purpose, isolated, short-term cultured monocytes from barrier-maintained adult cats of different ages (15 mo to 10 yr) were examined for transcription of IL-1 beta, IL-6, IL-10, IL-12 p40 and TNF-alpha by real-time PCR. Transcription levels of cytokines varied and were generally highest for IL-1 beta. For IL-1 beta, IL-6 and IL-12 p40, both young and old cats exhibited highest levels. The age association was significant. TNF-alpha appeared to be transcribed at similar levels over the examination period, whereas IL-10 tended to decline with age but without any statistical significant differences. The observed age association of the constitutive transcription of some cytokines indicates a drop in monocyte activities from youth to middle age, which is then followed by a (progressive) increase with increasing age. This provides evidence that monocytes are in part responsible for the pro-inflammatory status observed with ageing.
单核细胞/巨噬细胞是炎症和免疫的核心介质，因此在免疫衰老研究中备受关注。在健康成年猫中，单核细胞已被证明可组成性转录促炎和抗炎细胞因子。然而，为了表征年龄的影响，研究了猫单核细胞功能在免疫衰老早期细胞因子转录水平的变化。为此，通过实时PCR检测了从不同年龄（15个月至10岁）的屏障饲养成年猫中分离出的短期培养单核细胞的IL-1β、IL-6、IL-10、IL-12 p40和TNF-α转录情况。细胞因子的转录水平各不相同，IL-1β的转录水平通常最高。对于IL-1β、IL-6和IL-12 p40，年轻和老年猫均表现出最高水平。年龄相关性显著。在整个检测期间，TNF-α的转录水平似乎相似，而IL-10则随年龄增长呈下降趋势，但无统计学显著差异。观察到的某些细胞因子组成性转录与年龄的相关性表明，单核细胞活性从青年到中年有所下降，随后随着年龄增长（逐渐）增加。这提供了证据表明单核细胞在一定程度上导致了衰老过程中观察到的促炎状态。
