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快速目视检测法:快速可靠检测赭曲霉毒素 A。

Rapid visual tests: fast and reliable detection of ochratoxin A.

机构信息

Ecole des Mines d'Ales, 6 av. de Clavieres 30319, Ales cedex, France.

出版信息

Toxins (Basel). 2010 Sep;2(9):2230-41. doi: 10.3390/toxins2092230. Epub 2010 Aug 26.

Abstract

This paper reviews the early detection strategies that have been employed for the rapid monitoring of ochratoxin A (OTA) contamination of food. OTA, a mycotoxin mainly produced by some Aspergillus and Penicillium species, is found in cereals, coffee, wine, pork and grapes. To minimize the entry of this mycotoxin into the food chain, rapid diagnostic tools are required. To this end, the potential use of lateral flow devices has also been developed. In this study, we analyze the robustness of test strips using published methods for colorimetric detection. Different test formats are discussed, and challenges in the development of lateral flow devices for on-site determination of OTA, with requirements such as robustness, speed, and cost-effectiveness, are discussed.

摘要

本文综述了用于快速监测食品中赭曲霉毒素 A(OTA)污染的早期检测策略。OTA 是一种主要由某些曲霉属和青霉属产生的真菌毒素,存在于谷物、咖啡、葡萄酒、猪肉和葡萄中。为了最大限度地减少这种真菌毒素进入食物链,需要快速诊断工具。为此,也已经开发了侧向流动装置的潜在用途。在这项研究中,我们使用已发表的比色检测方法分析测试条的稳健性。讨论了不同的测试格式,并讨论了用于现场测定 OTA 的侧向流动装置的开发所面临的挑战,这些装置需要具有稳健性、速度和成本效益等要求。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3e/3153294/72e2a4f4d634/toxins-02-02230-g001.jpg

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