Gonda R, Takeda K, Shimizu N, Tomoda M
Kyoritsu College of Pharmacy, Tokyo, Japan.
Chem Pharm Bull (Tokyo). 1992 Jan;40(1):185-8. doi: 10.1248/cpb.40.185.
A neutral polysaccharide, named ukonan D, was isolated from the rhizome of Curcuma longa L. It produced a single band on electrophoresis and a single peak on gel chromatography, and its molecular mass was estimated to be 28,000. It showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test. Ukonan D is composed of L-arabinose: D-galactose: D-glucose: D-mannose in the molar ratio of 1:1:12:0.2, in addition to small amounts of peptide moiety. Methylation analysis, carbon-13 nuclear magnetic resonance and enzymic degradation studies indicated that its structural features include mainly both alpha-1,5-linked L-arabino-beta-3,6-branched D-galactan type and alpha-4,6-branched D-glucan type structural units. The influence of degradation with alpha-amylase followed by the elimination of glucan side chains on its immunological activity was discussed.
从姜黄的根茎中分离出一种名为ukonan D的中性多糖。它在电泳中呈现单一谱带，在凝胶色谱中呈现单一峰，其分子量估计为28,000。在碳清除试验中，它显示出显著的网状内皮系统增强活性。Ukonan D由L-阿拉伯糖：D-半乳糖：D-葡萄糖：D-甘露糖以1:1:12:0.2的摩尔比组成，此外还含有少量肽部分。甲基化分析、碳-13核磁共振和酶降解研究表明，其结构特征主要包括α-1,5-连接的L-阿拉伯糖-β-3,6-分支的D-半乳聚糖类型和α-4,6-分支的D-葡聚糖类型结构单元。讨论了用α-淀粉酶降解后消除葡聚糖侧链对其免疫活性的影响。
