Long Yun-fei, Chen Xiao-ming, Cai Tie-jun
Institute of Chemistry, Xiangtan University, Xiangtan 411105, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2004 May;24(5):610-2.
Characteristics of the interaction of nitro-tetrazolium chloride blue and Deoxyribonucleic Acid (DNA) were studied in the of pH 1.8 buffer solution by Resonance Light-Scattering (RLS) technique. The effects of interference substances and the optimization of the procedure were investigated, and a new method for the determination of the DNA was established based on RLS. The linear range of the DNA is 0-7 mg x L(-1) with a correlation coefficient of 0.9958. The detection limit is 30.04 microg x L(-1). The method has been applied to the determination of DNA in mixed samples with satisfactory result.
在pH 1.8的缓冲溶液中，采用共振光散射（RLS）技术研究了氯化硝基四氮唑蓝与脱氧核糖核酸（DNA）的相互作用特性。考察了干扰物质的影响及实验条件的优化，建立了基于RLS测定DNA的新方法。DNA的线性范围为0～7mg·L⁻¹，相关系数为0.9958，检出限为30.04μg·L⁻¹。该方法已用于混合样品中DNA的测定，结果令人满意。
