冷冻电子显微镜揭示二氧化硅纳米颗粒上支撑脂质双层的形成。

The formation of supported lipid bilayers on silica nanoparticles revealed by cryoelectron microscopy.

作者信息

Mornet Stéphane, Lambert Olivier, Duguet Etienne, Brisson Alain

机构信息

Molecular Imaging and Nano-Bio-Technology, IECB, UMR-CNRS 5471, University of Bordeaux 1, 2 rue Robert Escarpit, F-33607 Pessac, France.

出版信息

Nano Lett. 2005 Feb;5(2):281-5. doi: 10.1021/nl048153y.

DOI:10.1021/nl048153y

PMID:15794611

Abstract

The controlled fabrication of biocompatible devices made of lipid bilayers deposited onto flat solid supports presents interest as models of cell membranes as well as for their biotechnological applications. We report here on the formation of supported lipid bilayers on silica nanoparticles (nanoSLBs). The successive steps of the adsorption of lipid vesicles on nanoparticles and the formation of nanoSLBs are revealed in detail by cryotransmission electron microscopy (cryo-EM). The formation of nanoSLBs was achieved for liposomes with positive, neutral, and low net negative charge, while liposomes with a high net negative charge adsorbed to silica nanoparticles but did not rupture. The nanoSLBs were found to follow faithfully the surface contours of the particles, information yet unavailable for SLB formation on planar solid substrates.

摘要

将脂质双层沉积在平坦固体支持物上制成的生物相容性装置的可控制造，作为细胞膜模型及其生物技术应用具有重要意义。我们在此报告了在二氧化硅纳米颗粒上形成支持脂质双层（nanoSLB）的情况。通过低温透射电子显微镜（cryo-EM）详细揭示了脂质囊泡在纳米颗粒上的吸附以及nanoSLB形成的连续步骤。对于带正电荷、中性和低净负电荷的脂质体，成功实现了nanoSLB的形成，而具有高净负电荷的脂质体吸附到二氧化硅纳米颗粒上但未破裂。发现nanoSLB忠实地遵循颗粒的表面轮廓，而在平面固体基质上形成SLB时尚无法获得此类信息。