Ormen Oyvind, Granum Per Einar, Lassen Jørgen, Figueras Maria Jose
Department of Food Safety and Infection Biology, The Norwegian School of Veterinary Science, N-0033 Oslo, Norway.
APMIS. 2005 Mar;113(3):203-7. doi: 10.1111/j.1600-0463.2005.apm1130308.x.
Biochemical and genetic identification by RFLP (restriction fragment length polymorphism) of the PCR-amplified 16S r-RNA sequence were compared for a selection of 171 clinical and environmental isolates of Aeromonas spp. The investigation revealed large differences between the two methods. The species phenotypic identification scheme and the genetic technique applied to the environmental strains gave divergent results for 96% of the strains tested. There was 46% discrepancy between the two methods for the clinical isolates. The distribution of species differed between clinical and environmental isolates. A. hydrophila, A. caviae, A. jandaei and A. veronii dominated the clinical material (81% of isolates by RFLP), whilst only 21% of the environmental isolates belonged to those four species. From the environmental group A. salmonicida, A. bestiarum, A. sobria, A. media, and A. encheleia contributed 72% of the strains tested. The poor parity between the biochemical and the genetic identification of the environmental isolates, and to a lesser extent for the clinical isolates, underlines the fact that our current biochemical methods cannot adequately differentiate Aeromonas spp. This work also shows that the biochemical schemes derived from clinical isolates are incomplete for the identification of environmental strains.
对171株气单胞菌属的临床和环境分离株，比较了通过聚合酶链反应（PCR）扩增的16S r - RNA序列的限制性片段长度多态性（RFLP）进行生化和基因鉴定的结果。调查发现这两种方法存在很大差异。应用于环境菌株的种表型鉴定方案和基因技术，对96%的测试菌株给出了不同的结果。对于临床分离株，两种方法之间存在46%的差异。临床和环境分离株的菌种分布不同。嗜水气单胞菌、豚鼠气单胞菌、詹氏气单胞菌和维氏气单胞菌在临床样本中占主导地位（通过RFLP分析，81%的分离株属于这几种），而环境分离株中只有21%属于这四个菌种。在环境分离株中，生化鉴定和基因鉴定之间的一致性较差，临床分离株的一致性稍好，这突出表明我们目前的生化方法不能充分区分气单胞菌属。这项工作还表明，从临床分离株得出的生化鉴定方案对于环境菌株的鉴定是不完整的。
